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Literature summary for 7.3.2.7 extracted from
- Structure-function analysis of the ArsA ATPase: contribution of histidine residues (2001), J. Bioenerg. Biomembr., 33, 459-468.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 with a His-tag | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H138A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H148A | cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme | Escherichia coli |
| H219A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H327A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H359A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H368A | cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme | Escherichia coli |
| H388A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H397A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H453A | cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme | Escherichia coli |
| H465A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H477A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H520A | cells with this protein were resistant to arsenite and antimonite like wild-type | Escherichia coli |
| H558A | cells with this protein were resistant to arsenite and antimonite like wild-type, 50% lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | inhibition could be reversed upon subsequent incubation with hydroxylamine | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArsA ATPase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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