Cloned (Comment) | Organism |
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expression of His-tagged ModA in Escherichia coli strain BL21-Gold (DE3) | Methanosarcina acetivorans |
Crystallization (Comment) | Organism |
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ModA in the apoprotein conformation and in the molybdate-bound conformation, by sitting-drop and hanging-drop vapor-diffusion methods, mixing of 0.001 ml of protein solution containing 16-23 mg/ml protein with 0.001 ml reservoir solution, containing 1.4 M ammonium citrate pH 6.4 or 1.4 M ammonium citrate pH 6.0, 4 mM sodium sulfate for the apo-protein and 2.1 M ammonium sulfate and either 1% v/v or 3% v/v 2-propanol, and 4% v/v 2.4 M ammonium sulfate for the molybdate-bound protein, equilibration against 100 ml reservoir solution at room temperature for apo-odA and at 293 K for molybdate-bound ModA, 1 week, method optimization, X-ray diffraction structure determination and analysis of apo- and molybdate-bound-ModA at 1.95 A and 1.69 A resolution and at 2.25 A and 2.45 A resolutionm, respectively, molecular replacemen fails, usage of heavy metal labeling instead | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + molybdate/out | Methanosarcina acetivorans | - |
ADP + phosphate + molybdate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged ModA from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography and gel filtration | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + molybdate/out | - |
Methanosarcina acetivorans | ADP + phosphate + molybdate/in | - |
? | |
ATP + H2O + molybdate/out | molybdate-bound MaModA employs octahedral coordination of its substrate | Methanosarcina acetivorans | ADP + phosphate + molybdate/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | ModABC consists of the ModA periplasmic solute-binding protein, the integral membrane-transport protein ModB and the ATP-binding and hydrolysis cassette protein ModC. Bilobal domain structure of ModA with two mixed alpha/beta domains linked by a hinge region and with a deep cleft between the two domains. Upon binding ligand one domain is rotated towards the other by a hinge-bending motion analogously to the Venus flytrap model of bacterial-type periplasmic binding proteins | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
ModABC | - |
Methanosarcina acetivorans |
General Information | Comment | Organism |
---|---|---|
physiological function | ModABC consists of the ModA periplasmic solute-binding protein, the integral membrane-transport protein ModB and the ATP-binding and hydrolysis cassette protein ModC | Methanosarcina acetivorans |