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Literature summary for 7.2.2.10 extracted from
- The characterization of plasma membrane Ca2+-ATPase in rich sphingomyelin-cholesterol domains (2005), FEBS Lett., 579, 2397-2403.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Sus scrofa | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Sus scrofa |  |
| Mg2+ | required | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from brain by calmodulin affinity chromatography to homogeneity | Sus scrofa |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of purified enzyme into proteoliposomes using microbeads, the enzyme is mixed with lipid in a 1:5000 protein to lipid molar ratio, removal of detergent, sealing of vesicles | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ uptake activity into artificial proteoliposomes, overview | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | ATPase activity and Ca2+ uptake into artificial proteoliposomes activity | Sus scrofa | ADP + phosphate + Ca2+/trans | - |
? | |
| additional information | the enzyme binds calmodulin with high affinity | Sus scrofa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | PMCA is rich in sphingomyelin-cholesterol domains | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| plasma membrane Ca2+-ATPase | - |
Sus scrofa |
| PMCA | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Sus scrofa |
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Release 2023.1 (January 2023)
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