BRENDA - Enzyme Database show
show all sequences of 6.3.2.24

NG-nitro-L-arginine methyl ester and alpha-methyl-L-ornithine inhibit kyotorphin synthetase from rat brain

Kawabata, A.; Tanaka, M.; Muguruma, H.; Takagi, H.; Peptides 16, 1317-1319 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-methyl-L-ornithine
50% inhibition at 2.51 mM
Rattus norvegicus
NG-nitro-L-arginine methyl ester
50% inhibition at 2.33 mM
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
245000
gel filtration
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-tyrosine + L-arginine
-
648949
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
-
648949
Rattus norvegicus
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-methyl-L-ornithine
50% inhibition at 2.51 mM
Rattus norvegicus
NG-nitro-L-arginine methyl ester
50% inhibition at 2.33 mM
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
245000
gel filtration
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-tyrosine + L-arginine
-
648949
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
-
648949
Rattus norvegicus
?
Other publictions for EC 6.3.2.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745971
Tsukahara
Tyrosyl-tRNA synthetase A pot ...
Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Peptides
101
60-68
2018
-
-
1
-
-
-
-
3
-
1
-
-
-
145
-
-
1
-
-
19
-
-
8
1
3
1
-
-
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
19
-
-
8
1
3
1
-
-
1
1
-
-
-
5
5
-
-
-
648948
Kawabata
Kyotorphin synthetase activity ...
Rattus norvegicus
Peptides
17
407-411
1996
-
-
-
-
-
-
4
5
1
1
1
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648949
Kawabata
NG-nitro-L-arginine methyl est ...
Rattus norvegicus
Peptides
16
1317-1319
1995
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648947
Ueda
Kyotorphin (tyrosine-arginine) ...
Rattus norvegicus
J. Biol. Chem.
262
8165-8173
1987
-
-
-
-
-
-
6
4
2
2
1
1
-
2
-
-
1
-
-
1
1
-
14
-
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
4
2
2
1
1
-
-
-
1
-
1
1
-
14
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-