Information on EC 6.3.2.24 - tyrosine-arginine ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.2.24
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RECOMMENDED NAME
GeneOntology No.
tyrosine-arginine ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-tyrosine + L-arginine = AMP + diphosphate + L-tyrosyl-L-arginine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptide synthase reaction
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SYSTEMATIC NAME
IUBMB Comments
L-tyrosine:L-arginine ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
116036-78-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
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37.8% of activity compared to ATP
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AMP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
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1,6% of activity compared to ATP
?
ATP + L-arginine + leucine
? + AMP
show the reaction diagram
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3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + lysine
? + AMP
show the reaction diagram
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10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + phenylalanine
? + AMP
show the reaction diagram
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10% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + tryptophan
? + AMP
show the reaction diagram
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11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + glutamate
? + AMP
show the reaction diagram
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26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + glycine
? + AMP
show the reaction diagram
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1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + histidine
? + AMP
show the reaction diagram
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4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + L-arginine
AMP + diphosphate + L-tyrosyl-L-arginine
show the reaction diagram
ATP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + AMP + diphosphate
show the reaction diagram
ATP + L-tyrosine + leucine
? + AMP
show the reaction diagram
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31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + lysine
? + AMP
show the reaction diagram
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14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
GTP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
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20.2% of activity compared to ATP
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + AMP + diphosphate
show the reaction diagram
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involved in biosynthesis of kyotorphin in rat brain
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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102,7% of activity compared to Mg2+, may substitute for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-methyl-L-ornithine
Co2+
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0.4-4 mM: strong
Cu2+
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0.04-4 mM: strong
D-arginine
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diphosphate
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10 mM: 97.6% inhibition
K+
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50-100 mM: strong
NG-nitro-L-arginine methyl ester
NH4+
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10-100 mM: strong
phosphate
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10 mM: 4.2% inhibition
additional information
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not inhibitory: NG-nitro-L-arginine, N-iminoethyl-L-ornithine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 0.294
ATP
0.926 - 7.83
L-arginine
0.0256 - 0.2
L-tyrosine
0.442
MgCl2
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37°C, pH 8.0
additional information
additional information
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Michaelis-Menten kinetics
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000016
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37°C, pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
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broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.2 - 9.5
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KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2
8 - 10
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28% of maximal activity at pH 8, 85% of maximal activity at pH 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 70
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60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Manually annotated by BRENDA team
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; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Manually annotated by BRENDA team
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; highest expression level
Manually annotated by BRENDA team
additional information
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expression in all organs tested
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
200000 - 300000
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gel filtration, both enzyme from adrenal gland as well as from spinal cord
240000 - 245000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 59000, recombinant His6-tagged enzyme, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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