BRENDA - Enzyme Database show
show all sequences of 6.3.2.24

Kyotorphin (tyrosine-arginine) synthetase in rat brain synaptosomes

Ueda, H.; Yoshihara, Y.; Fukushima, N.; Shiomi, H.; Nakamura, A.; Takagi, H.; J. Biol. Chem. 262, 8165-8173 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
0.4-4 mM: strong
Rattus norvegicus
Cu2+
0.04-4 mM: strong
Rattus norvegicus
diphosphate
10 mM: 97.6% inhibition
Rattus norvegicus
K+
50-100 mM: strong
Rattus norvegicus
NH4+
10-100 mM: strong
Rattus norvegicus
phosphate
10 mM: 4.2% inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0256
-
L-tyrosine
37°C, pH 8.0
Rattus norvegicus
0.294
-
ATP
37°C, pH 8.0
Rattus norvegicus
0.442
-
MgCl2
37°C, pH 8.0
Rattus norvegicus
0.926
-
L-arginine
37°C, pH 8.0
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rattus norvegicus
-
-
synaptosome
highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction
Rattus norvegicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement; Km: 0.442 mM, MgCl2
Rattus norvegicus
Mn2+
102,7% of activity compared to Mg2+, may substitute for Mg2+
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
245000
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-tyrosine + L-arginine
Rattus norvegicus
involved in biosynthesis of kyotorphin in rat brain
L-tyrosyl-L-arginine + AMP + diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000016
-
37°C, pH 8.0
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
37.8% of activity compared to ATP
648947
Rattus norvegicus
-
AMP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
1,6% of activity compared to ATP
648947
Rattus norvegicus
?
ATP + L-arginine + leucine
-
648947
Rattus norvegicus
? + AMP
3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + lysine
-
648947
Rattus norvegicus
? + AMP
10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + phenylalanine
-
648947
Rattus norvegicus
? + AMP
10% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + tryptophan
-
648947
Rattus norvegicus
? + AMP
11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + glutamate
-
648947
Rattus norvegicus
? + AMP
26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + glycine
-
648947
Rattus norvegicus
? + AMP
1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + histidine
-
648947
Rattus norvegicus
? + AMP
4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
i.e. kyotorphin
648947
Rattus norvegicus
?
ATP + L-tyrosine + L-arginine
involved in biosynthesis of kyotorphin in rat brain
648947
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
-
-
-
?
ATP + L-tyrosine + leucine
-
648947
Rattus norvegicus
? + AMP
31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + lysine
-
648947
Rattus norvegicus
? + AMP
14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
GTP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
20.2% of activity compared to ATP
648947
Rattus norvegicus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
broad
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10
28% of maximal activity at pH 8, 85% of maximal activity at pH 10
Rattus norvegicus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
isoelectric focusing
-
6.1
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
0.4-4 mM: strong
Rattus norvegicus
Cu2+
0.04-4 mM: strong
Rattus norvegicus
diphosphate
10 mM: 97.6% inhibition
Rattus norvegicus
K+
50-100 mM: strong
Rattus norvegicus
NH4+
10-100 mM: strong
Rattus norvegicus
phosphate
10 mM: 4.2% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0256
-
L-tyrosine
37°C, pH 8.0
Rattus norvegicus
0.294
-
ATP
37°C, pH 8.0
Rattus norvegicus
0.442
-
MgCl2
37°C, pH 8.0
Rattus norvegicus
0.926
-
L-arginine
37°C, pH 8.0
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rattus norvegicus
-
-
synaptosome
highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction
Rattus norvegicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement; Km: 0.442 mM, MgCl2
Rattus norvegicus
Mn2+
102,7% of activity compared to Mg2+, may substitute for Mg2+
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
245000
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-tyrosine + L-arginine
Rattus norvegicus
involved in biosynthesis of kyotorphin in rat brain
L-tyrosyl-L-arginine + AMP + diphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000016
-
37°C, pH 8.0
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
37.8% of activity compared to ATP
648947
Rattus norvegicus
-
AMP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
1,6% of activity compared to ATP
648947
Rattus norvegicus
?
ATP + L-arginine + leucine
-
648947
Rattus norvegicus
? + AMP
3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + lysine
-
648947
Rattus norvegicus
? + AMP
10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + phenylalanine
-
648947
Rattus norvegicus
? + AMP
10% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-arginine + tryptophan
-
648947
Rattus norvegicus
? + AMP
11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + glutamate
-
648947
Rattus norvegicus
? + AMP
26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + glycine
-
648947
Rattus norvegicus
? + AMP
1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + histidine
-
648947
Rattus norvegicus
? + AMP
4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
i.e. kyotorphin
648947
Rattus norvegicus
?
ATP + L-tyrosine + L-arginine
involved in biosynthesis of kyotorphin in rat brain
648947
Rattus norvegicus
L-tyrosyl-L-arginine + AMP + diphosphate
-
-
-
?
ATP + L-tyrosine + leucine
-
648947
Rattus norvegicus
? + AMP
31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
ATP + L-tyrosine + lysine
-
648947
Rattus norvegicus
? + AMP
14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
648947
Rattus norvegicus
?
GTP + L-tyrosine + L-arginine
-
648947
Rattus norvegicus
L-tyrosyl-L-arginine + ?
20.2% of activity compared to ATP
648947
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
broad
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10
28% of maximal activity at pH 8, 85% of maximal activity at pH 10
Rattus norvegicus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
isoelectric focusing
-
6.1
Other publictions for EC 6.3.2.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745971
Tsukahara
Tyrosyl-tRNA synthetase A pot ...
Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Peptides
101
60-68
2018
-
-
1
-
-
-
-
3
-
1
-
-
-
145
-
-
1
-
-
19
-
-
8
1
3
1
-
-
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
19
-
-
8
1
3
1
-
-
1
1
-
-
-
5
5
-
-
-
648948
Kawabata
Kyotorphin synthetase activity ...
Rattus norvegicus
Peptides
17
407-411
1996
-
-
-
-
-
-
4
5
1
1
1
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648949
Kawabata
NG-nitro-L-arginine methyl est ...
Rattus norvegicus
Peptides
16
1317-1319
1995
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648947
Ueda
Kyotorphin (tyrosine-arginine) ...
Rattus norvegicus
J. Biol. Chem.
262
8165-8173
1987
-
-
-
-
-
-
6
4
2
2
1
1
-
2
-
-
1
-
-
1
1
-
14
-
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
4
2
2
1
1
-
-
-
1
-
1
1
-
14
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-