Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.4-4 mM: strong | Rattus norvegicus | |
Cu2+ | 0.04-4 mM: strong | Rattus norvegicus | |
diphosphate | 10 mM: 97.6% inhibition | Rattus norvegicus | |
K+ | 50-100 mM: strong | Rattus norvegicus | |
NH4+ | 10-100 mM: strong | Rattus norvegicus | |
phosphate | 10 mM: 4.2% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0256 | - |
L-tyrosine | 37°C, pH 8.0 | Rattus norvegicus | |
0.294 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus | |
0.442 | - |
MgCl2 | 37°C, pH 8.0 | Rattus norvegicus | |
0.926 | - |
L-arginine | 37°C, pH 8.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
synaptosome | highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Rattus norvegicus | |
Mg2+ | Km: 0.442 mM, MgCl2 | Rattus norvegicus | |
Mn2+ | 102,7% of activity compared to Mg2+, may substitute for Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
240000 | 245000 | gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + L-arginine | Rattus norvegicus | involved in biosynthesis of kyotorphin in rat brain | L-tyrosyl-L-arginine + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000016 | - |
37°C, pH 8.0 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + L-tyrosine + L-arginine | - |
Rattus norvegicus | L-tyrosyl-L-arginine + ? | 37.8% of activity compared to ATP | ? | |
AMP + L-tyrosine + L-arginine | - |
Rattus norvegicus | L-tyrosyl-L-arginine + ? | 1,6% of activity compared to ATP | ? | |
ATP + L-arginine + leucine | - |
Rattus norvegicus | ? + AMP | 3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-arginine + lysine | - |
Rattus norvegicus | ? + AMP | 10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-arginine + phenylalanine | - |
Rattus norvegicus | ? + AMP | 10% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-arginine + tryptophan | - |
Rattus norvegicus | ? + AMP | 11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-tyrosine + glutamate | - |
Rattus norvegicus | ? + AMP | 26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-tyrosine + glycine | - |
Rattus norvegicus | ? + AMP | 1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-tyrosine + histidine | - |
Rattus norvegicus | ? + AMP | 4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-tyrosine + L-arginine | - |
Rattus norvegicus | L-tyrosyl-L-arginine + AMP + diphosphate | i.e. kyotorphin | ? | |
ATP + L-tyrosine + L-arginine | involved in biosynthesis of kyotorphin in rat brain | Rattus norvegicus | L-tyrosyl-L-arginine + AMP + diphosphate | - |
? | |
ATP + L-tyrosine + leucine | - |
Rattus norvegicus | ? + AMP | 31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
ATP + L-tyrosine + lysine | - |
Rattus norvegicus | ? + AMP | 14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine | ? | |
GTP + L-tyrosine + L-arginine | - |
Rattus norvegicus | L-tyrosyl-L-arginine + ? | 20.2% of activity compared to ATP | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | broad | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | 28% of maximal activity at pH 8, 85% of maximal activity at pH 10 | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectric focusing | - |
6.1 |