Cloned (Comment) | Organism |
---|---|
gene tryS, recombinant expression in Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-(S,R)-buthionine sulfoximine | DL-(S,R)-BSO, a multitarget inhibitor of trypanothione synthesis, in vivo effects on nonthiol-supplied epimastigote cells, on Cys-supplied epimastigote cells, and on GSH-supplied epimastigote cells, overview. Inhibition of gamma-glutamylcysteine synthetase and trypanothione synthetase. Wild-type Trypanosoma brucei cells are not restored by supplementing with 0.08 mM GSH. The the L-(S,R)-BSO stereoisomer is more potent that the the D-(S,R)-BSO. Inhibitor docking analysis, binding structure | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + glutathionylspermidine + ATP | Trypanosoma cruzi | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | Trypanosoma cruzi DTU I | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Trypanosoma cruzi | - |
glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Trypanosoma cruzi DTU I | - |
glutathionylspermidine + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi DTU I | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + glutathionylspermidine + ATP | - |
Trypanosoma cruzi | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | - |
Trypanosoma cruzi DTU I | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | - |
Trypanosoma cruzi | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | - |
Trypanosoma cruzi DTU I | glutathionylspermidine + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TcTryS | - |
Trypanosoma cruzi |
TryS | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
additional information | docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview | Trypanosoma cruzi |