Cloned (Comment) | Organism |
---|---|
gene gspS, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Crithidia fasciculata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phosphinate | behaves as a slow-binding bisubstrate inhibitor, competitive with respect to GSH and spermidine | Crithidia fasciculata | |
phosphinate analogue of glutathionylspermidine | slow tight-binding inhibition | Crithidia fasciculata | |
phosphonate | acts as a a simple ground state analogue of glutathione | Crithidia fasciculata | |
phosphonate analogue of glutathionylspermidine | classical, linear competitive inhibition with respect to GSH | Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.157 | - |
spermidine | pH 7.2, 25°C | Crithidia fasciculata | |
0.215 | - |
ATP | pH 7.2, 25°C | Crithidia fasciculata | |
0.609 | - |
glutathione | pH 7.2, 25°C | Crithidia fasciculata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Crithidia fasciculata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + spermidine + ATP | Crithidia fasciculata | glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione, an essential metabolite in defence against chemical and oxidative stress in the kinetoplastida | glutathionylspermidine + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | P90518 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to over 98% purity | Crithidia fasciculata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate | the kinetic mechanism obeys a rapid equilibrium random ter-ter model | Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + spermidine + ATP | - |
Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione, an essential metabolite in defence against chemical and oxidative stress in the kinetoplastida | Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
additional information | model of ter-reactant mechanism of GspS catalysis and postulated overview | Crithidia fasciculata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GspS | - |
Crithidia fasciculata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Crithidia fasciculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Crithidia fasciculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Crithidia fasciculata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics and mechanism, model of ter-reactant mechanism of GspS catalysis and postulated, overview | Crithidia fasciculata | |
0.019 | - |
phosphinate analogue of glutathionylspermidine | pH 7.2, 25°C | Crithidia fasciculata | |
0.029 | - |
Phosphinate | pH 7.2, 25°C | Crithidia fasciculata | |
0.156 | - |
phosphonate analogue of glutathionylspermidine | pH 7.2, 25°C | Crithidia fasciculata |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.072 | - |
pH 7.2, 25°C | Crithidia fasciculata | Phosphinate |