Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Reconstitution of the heterodimeric active reverse gyrase from the two recombinant proteins overexpressed in Escherichia coli and purification. Subunit RgyB has a DNA-dependent ATPase activity at high temperature (80 °C) and is independent of the presence of subunit RgyA. Subunit RgyA alone has no detectable activity. The addition of subunit RgyA to subunit RgyB reconstitutes positive supercoiling activity | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
138000 | - |
1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | reconstitution of the heterodimeric active reverse gyrase from the two recombinant proteins overexpressed in Escherichia coli and purification. Subunit RgyB has a DNA-dependent ATPase activity at high temperature (80 °C) and is independent of the presence of subunit RgyA. Subunit RgyA alone has no detectable activity. The addition of subunit RgyA to subunit RgyB reconstitutes positive supercoiling activity | Methanopyrus kandleri | ? | - |
? | |
negatively supercoiled DNA + ATP + H2O | - |
Methanopyrus kandleri | positively supercoiled DNA + ADP + phosphate | - |
? | |
relaxed DNA + ATP + H2O | - |
Methanopyrus kandleri | positively supercoiled DNA + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent type I 5'-topoisomerase | - |
Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanopyrus kandleri |