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Literature summary for 5.6.2.2 extracted from

  • Graille, M.; Cladiere, L.; Durand, D.; Lecointe, F.; Gadelle, D.; Quevillon-Cheruel, S.; Vachette, P.; Forterre, P.; van Tilbeurgh, H.
    Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms (2008), Structure, 16, 360-370.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharolobus shibatae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in complex with the radicicol inhibitor Saccharolobus shibatae

Inhibitors

Inhibitors Comment Organism Structure
radicicol
-
 Saccharolobus shibatae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 derived from amino acid sequence Saccharolobus shibatae
220000
-
 gel filtration Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and heparin column chromatography Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA + ATP + H2O DNA cleavage by topo VI generates two-nucleotide 5'-protruding ends Saccharolobus shibatae relaxed DNA + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
heterotetramer x-ray crystallography Saccharolobus shibatae

Synonyms

Synonyms Comment Organism
DNA topoisomerase VI
-
 Saccharolobus shibatae
topo VI
-
 Saccharolobus shibatae

Cofactor

Cofactor Comment Organism Structure
ATP topo VI strictly requires ATP for DNA cleavage Saccharolobus shibatae