Literature summary for 5.6.2.2 extracted from

Chene, P.; Rudloff, J.; Schoepfer, J.; Furet, P.; Meier, P.; Qian, Z.; Schlaeppi, J.M.; Schmitz, R.; Radimerski, T.
Catalytic inhibition of topoisomerase II by a novel rationally designed ATP-competitive purine analogue (2009), BMC Chem. Biol., 9, 1-16.

Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
topoisomerase II alpha is expressed in Sf21 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
doxorubicin	-	Homo sapiens
ICRF-193	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap HP affinity column chromatography, StrepTactin column chromatography, and SPX200 gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
breast cancer cell	with a mutation in gene BRCA1	Homo sapiens	-
HCC-1937 cell	-	Homo sapiens	-
HL-60 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
supercoiled DNA + ATP + H2O	-	Homo sapiens	catenated DNA networks + ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Topo IIalpha	isoform	Homo sapiens
Topo IIbeta	isoform	Homo sapiens
Topoisomerase II	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0002	-	at 37°C	Homo sapiens	ICRF-193

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Release 2023.1 (January 2023)