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Literature summary for 5.6.2.2 extracted from
- DNA topoisomerase II selects DNA cleavage sites based on reactivity rather than binding affinity (2007), Nucleic Acids Res., 35, 3764-3773.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of full-length Saccharomyces cerevisiae topoisomerase II fused to an intein and a chitin binding domain affinity tag in the yeast strain BCY123 | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the sequence that defines a cleavage site resides within the central 20 bp of a dNA duplex. The DNA affinity does not correlate with the ability of the enzyme to cleave DNA. The binding step does not contribute significantly to the selection mechanism | Saccharomyces cerevisiae | ? | - |
? |  |
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