Literature summary for 5.6.2.2 extracted from

Carr, S.B.; Makris, G.; Phillips, S.E.; Thomas, C.D.
Crystallization and preliminary X-ray diffraction analysis of two N-terminal fragments of the DNA-cleavage domain of topoisomerase IV from Staphylococcus aureus (2006), Acta Crystallogr. Sect. F, 62, 1164-1167.

Application

Application	Comment	Organism
pharmacology	Topo II are attractive targets for design of antitumor agents	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
Protein expression performed in Escherichia coli B834 DE3 (plysS)	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
first report of crystallization and preliminary X-ray analysis of the DNA-cleavage domain of a topoisomerase IV from a gram positive organism. Sparse-matrix screening used, Crystals of both protein fragments (GrlA56 and GrlA59) display a plate-like morphology and initially grow in tightly packed clusters. Crystallization and X-ray analysis of two different constructs of the A subunit of topoisomerase IV from Staphylococcus aureus (GrlA) are described. With a view to gaining further insight into the mode of inhibition of quinolone antibiotics against this enzyme	Staphylococcus aureus

Crystallization (Comment)

Organism

first report of crystallization and preliminary X-ray analysis of the DNA-cleavage domain of a topoisomerase IV from a gram positive organism. Sparse-matrix screening used, Crystals of both protein fragments (GrlA56 and GrlA59) display a plate-like morphology and initially grow in tightly packed clusters. Crystallization and X-ray analysis of two different constructs of the A subunit of topoisomerase IV from Staphylococcus aureus (GrlA) are described. With a view to gaining further insight into the mode of inhibition of quinolone antibiotics against this enzyme

Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	generated protein GrlA56	Staphylococcus aureus
59000	-	generated protein GrlA59	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q6G9K4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Subunits

Subunits	Comment	Organism
heterotetramer	Two constructs of 56 and 59 kDa spanning the DNA-cleavage domain of the A subunit of topoisomerase IV from Staphylococcus aureus. All bacterial type IIA topoisomerases are A2B2 heterotetramers containing three discrete subunit interfaces which open and close sequentially in response to ATP binding and hydrolysis in order to effect DNA transport	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
DNA topoisomerase IV	type IIA DNA topoisomerase	Staphylococcus aureus
topoIV	-	Staphylococcus aureus