Literature summary for 5.6.2.2 extracted from

Inoue, Y.; Sato, K.; Fujii, T.; Hirai, K.; Inoue, M.; Iyobe, S.; Mitsuhashi, S.
Some properties of subunits of DNA gyrase from Pseudomonas aeruginosa PAO1 and its nalidixic acid-resistant mutant (1987), J. Bacteriol., 169, 2322-2325.

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Protein Variants

Protein Variants	Comment	Organism
F116L	naturally occurring nalidixic acid-resistant mutant strain 10	Pseudomonas aeruginosa
additional information	construction of reconstituted enzymes consisting of wild-type subunit A and mutant subunit B and vice versa, the reconstituted enzymes with wild-type subunit A show wild-type inhibitor sensitivity, the ones with mutant subunit A show mutant inhibitor sensitivity	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
Ciprofloxacin	IC50 for the wild-type enzyme is 0.008 mg/ml and 0.4 mg/ml for the mutant 10 enzyme	Pseudomonas aeruginosa
Nalidixic acid	IC50 for the wild-type enzyme and the mutant 10 enzyme is above 0.4 mg/ml, inhibits both the replication of DNA in growing cells and the supercoiling enzyme activity in vitro	Pseudomonas aeruginosa
Norfloxacin	IC50 for the wild-type enzyme is 0.017 mg/ml and 0.4 mg/ml for the mutant 10 enzyme	Pseudomonas aeruginosa
novobiocin	IC50 for the wild-type enzyme is 0.0002 mg/ml and 0.0004 mg/ml for the mutant 10 enzyme	Pseudomonas aeruginosa
ofloxacin	IC50 for the wild-type enzyme is 0.018 mg/ml and 0.4 mg/ml for the mutant 10 enzyme	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	divalent cation is required for activity, at 2-20 mM	Pseudomonas aeruginosa
Mg2+	divalent cation is required for activity	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	strain PAO1 and a nalidixic acid-resistant mutant strain 10, genes nalA and nalB	-

Purification (Commentary)

Purification (Comment)	Organism
subunits A and B from strain PAO1 and from the nalidixic acid-resistant mutant strain 10 by ammonium sulfate fractionation, dialysis, and heparin and novobiocin affinity chromatography	Pseudomonas aeruginosa

Subunits

Subunits	Comment	Organism
tetramer	alpha2beta2	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
DNA gyrase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	required for activity	Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)