Inhibitors | Comment | Organism | Structure |
---|---|---|---|
etoposide | anti-cancer drug, the enzyme inhibition by etoposide is not influenced by the divalent cation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slightly activating above 5 mM | Homo sapiens | |
Ca2+ | activating at 1-5 mM | Homo sapiens | |
Cd2+ | activates up to 1 mM, inhibits above 1 mM | Homo sapiens | |
Co2+ | highly activating at 1-5 mM, enhances DNA cleavage mediated by topoisomerase II alpha in vitro and in cultured cells, 6-13fold higher activity in DNA cleavage compared to Mg2+, but reduced DNA ligation activity | Homo sapiens | |
Mg2+ | slightly activating above 5 mM | Homo sapiens | |
Mn2+ | highly activating at 1-5 mM | Homo sapiens | |
additional information | activity with divalent cations, overview, the enzyme inhibition by etoposide is not influenced by the divalent cation | Homo sapiens | |
Sr2+ | slightly activating above 5 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + ATP + H2O | Homo sapiens | cleavage, relaxation, decatenation, and religation of DNA | DNA + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | breast adenocarcinoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + ATP + H2O | cleavage, relaxation, decatenation, and religation of DNA | Homo sapiens | DNA + ADP + phosphate | - |
? | |
kinetoplast DNA + ATP + H2O | decatenation of kinetoplast DNA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
topoisomerase II alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |