Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-pyrimidin-7,8-benzoflavone | induces significant conformational changes of the isolated NBD1/NBD2 dimer in solution. 2-pyrimidin-7,8-benzoflavone does not modify the ATP binding constant, but reduces the ATP hydrolysis activity of the NBD1/NBD2 mixture. In absence of ATP, the NBD1/NBD2 dimer is disrupted by the compound, but in the presence of 2 mM ATP, the two NBDs keep dimerised, and a major change in the size and the shape of the structure is observed | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of recombinant NBD1, from residue 394 to residue 672, and NBD2, from residue 1191 to residue 1480, polypeptides in Escherichia coli | Homo sapiens |
General Stability | Organism |
---|---|
in the absence of ATP, the Gd-HCl concentration to denature half of the proteins is about 2.2 M. A smaller Gd-HCl concentration,1.4 M, is needed to produce the same effect upon the application of 25 nM of 2-pyrimidin-7,8-benzoflavone | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00331 | - |
ATP | isolated NBD1/NBD2 mixture, pH 8.0, 25°C | Homo sapiens | |
0.0071 | - |
ATP | isolated NBD1/NBD2 mixture, presence of 12 nM 2-pyrimidin-7,8-benzoflavone, pH 8.0, 25°C | Homo sapiens | |
0.00964 | - |
ATP | isolated NBD1/NBD2 mixture, presence of 25 nM 2-pyrimidin-7,8-benzoflavone, pH 8.0, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + closed Cl- channel | - |
Homo sapiens | ADP + phosphate + open Cl- channel | - |
? |