Cloned (Comment) | Organism |
---|---|
C terminally deca-histidine-tagged wild type human CFTR epxressed in baby hamster kidney-21 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
by hanging drop method, at 20 A resolution in the xy plane, at 30 A resolution along z plane, two crystal forms, one with a roughly hexagonal profile, the other with an opened-out triangular profile, the two conformations are in presence of a nucleotide, which may be related to the role of CFTR as an ion channel rather than a transporter, and may represent the open and closed states of the channel | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA matrix and subsequent WGA-agorse affinity step | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
with ATP as substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + closed Cl- channel | - |
Homo sapiens | ADP + phosphate + open Cl- channel | - |
? | |
GTP + H2O + closed Cl- channel | - |
Homo sapiens | GDP + phosphate + open Cl- channel | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CFTR | - |
Homo sapiens |
cystic fibrosis transmembrane conductance regulator | - |
Homo sapiens |