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Literature summary for 5.6.1.6 extracted from

  • Annereau, J.P.; Ko, Y.H.; Pedersen, P.L.
    Cystic fibrosis transmembrane conductance regulator: the NBF1+R (nucleotide-binding fold 1 and regulatory domain) segment acting alone catalyses a Co2+/Mn2+/Mg2+-ATPase activity markedly inhibited by both Cd2+ and the transition-state analogue orthovanadate (2003), Biochem. J., 371, 451-462.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.6

Protein Variants

Protein Variants Comment Organism
K464A mutant of the NBF1+R segment (nucleotide binding domain 1 and regulatory domain), Vmax is reduced about 50% Homo sapiens
K464H mutant of the NBF1+R segment (nucleotide binding domain 1 and regulatory domain), Vmax is reduced about 50% Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Homo sapiens
vanadate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 ATP 37°C, reaction with the the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ best divalent metal activator Homo sapiens
Mg2+ activates Homo sapiens
Mn2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + closed Cl- channel Homo sapiens
-
 ADP + phosphate + open Cl- channel
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) and its mutant forms K464H and K464A Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
the guanidine hydrochloride-solubilized and denatured wild-type and mutant NBF1+R proteins can be renatured at 25°C upon rapid dilution with a buffered solution containing glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + closed Cl- channel
-
 Homo sapiens ADP + phosphate + open Cl- channel
-
 ?
ATP + H2O + closed Cl- channel the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) has the capacity to catalyze ATP hydrolysis in the absence of a fusion partner Homo sapiens ADP + phosphate + open Cl- channel
-
 ?