Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells | Salvia divinorum |
Protein Variants | Comment | Organism |
---|---|---|
F255A/N313A | the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol | Salvia divinorum |
F255H | the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product | Salvia divinorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia divinorum | A0A1L3THX5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography and Superdex 200 gel filtration | Salvia divinorum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trichome | specific expression in trichomes | Salvia divinorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Salvia divinorum | (-)-kolavenol | - |
? | |
geranylgeranyl diphosphate | the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate | Salvia divinorum | clerodienyl diphosphate + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
clerodienyl diphosphate synthase | - |
Salvia divinorum |
CLPP synthase | - |
Salvia divinorum |
CPS2 | - |
Salvia divinorum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the committed step in salvinorin A biosynthesis | Salvia divinorum |