BRENDA - Enzyme Database show
show all sequences of 5.5.1.B7

Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase

Pelot, K.A.; Mitchell, R.; Kwon, M.; Hagelthorn, D.M.; Wardman, J.F.; Chiang, A.; Bohlmann, J.; Ro, D.K.; Zerbe, P.; Plant J. 89, 885-897 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells
Salvia divinorum
Engineering
Amino acid exchange
Commentary
Organism
F255A/N313A
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol
Salvia divinorum
F255H
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product
Salvia divinorum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salvia divinorum
A0A1L3THX5
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-affinity column chromatography and Superdex 200 gel filtration
Salvia divinorum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
specific expression in trichomes
Salvia divinorum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeranyl diphosphate
-
741179
Salvia divinorum
(-)-kolavenol
-
-
-
?
geranylgeranyl diphosphate
the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate
741179
Salvia divinorum
clerodienyl diphosphate + H+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells
Salvia divinorum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F255A/N313A
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol
Salvia divinorum
F255H
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product
Salvia divinorum
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-affinity column chromatography and Superdex 200 gel filtration
Salvia divinorum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
specific expression in trichomes
Salvia divinorum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeranyl diphosphate
-
741179
Salvia divinorum
(-)-kolavenol
-
-
-
?
geranylgeranyl diphosphate
the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate
741179
Salvia divinorum
clerodienyl diphosphate + H+
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the committed step in salvinorin A biosynthesis
Salvia divinorum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the committed step in salvinorin A biosynthesis
Salvia divinorum
Other publictions for EC 5.5.1.B7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741179
Pelot
Biosynthesis of the psychotrop ...
Salvia divinorum
Plant J.
89
885-897
2017
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