Information on EC 5.5.1.B7 - clerodienyl diphosphate synthase

for references in articles please use BRENDA:EC5.5.1.B7
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The expected taxonomic range for this enzyme is: Salvia divinorum

EC NUMBER
COMMENTARY hide
5.5.1.B7
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
clerodienyl diphosphate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate = (-)-kolavenol
show the reaction diagram
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl diphosphate-lyase (cyclizing, (-)-kolavenol-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme catalyzes the committed step in salvinorin A biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
(-)-kolavenol
show the reaction diagram
-
-
-
?
geranylgeranyl diphosphate
clerodienyl diphosphate + H+
show the reaction diagram
the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate
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-
?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
specific expression in trichomes
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-affinity column chromatography and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F255A/N313A
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol
F255H
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product