BRENDA - Enzyme Database show
show all sequences of 5.4.99.25

Identification of determinants in the protein partners aCBF5 and aNOP10 necessary for the tRNA:Psi55-synthase and RNA-guided RNA:PSI-synthase activities

Muller, S.; Fourmann, J.B.; Loegler, C.; Charpentier, B.; Branlant, C.; Nucleic Acids Res. 35, 5610-5624 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
protein aNOP10
interaction with the pseudouridine synthase aCBF5 is required for the RNA-guided RNA:pseudouridine-synthase activity. The stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence. Nonetheless, interaction of aNOP10 with aCBF5 can counterbalance the absence of the PUA domain or the CCA sequence and more generally helps the aCBF5 tRNA:pseudouridine55-synthase activity. Substitution of the aNOP10 residue Y14 by an alanine disturbs this activity, it only impairs mildly the RNA-guided activity. The opposite effect is observed for the aNOP10 variant H31A
Pyrococcus abyssi
Engineering
Amino acid exchange
Commentary
Organism
K53A
substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities
Pyrococcus abyssi
R202A
substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities
Pyrococcus abyssi
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus abyssi
Q9V1A5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tRNA uridine55
the stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence
705960
Pyrococcus abyssi
tRNA pseudouridine55
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
protein aNOP10
interaction with the pseudouridine synthase aCBF5 is required for the RNA-guided RNA:pseudouridine-synthase activity. The stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence. Nonetheless, interaction of aNOP10 with aCBF5 can counterbalance the absence of the PUA domain or the CCA sequence and more generally helps the aCBF5 tRNA:pseudouridine55-synthase activity. Substitution of the aNOP10 residue Y14 by an alanine disturbs this activity, it only impairs mildly the RNA-guided activity. The opposite effect is observed for the aNOP10 variant H31A
Pyrococcus abyssi
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K53A
substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities
Pyrococcus abyssi
R202A
substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities
Pyrococcus abyssi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tRNA uridine55
the stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence
705960
Pyrococcus abyssi
tRNA pseudouridine55
-
-
-
?
Other publictions for EC 5.4.99.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748425
Fitzek
Evolution of eukaryal and arc ...
Homo sapiens, Methanocaldococcus jannaschii
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86
77-89
2018
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2
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1
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5
5
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730510
Friedt
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Escherichia coli
Nucleic Acids Res.
42
3857-3870
2014
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728620
Fourmann
Comparative study of two box H ...
Pyrococcus abyssi
PLoS One
8
e70313
2013
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1
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728787
Joardar
Role of forefinger and thumb l ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
RNA
19
1279-1294
2013
-
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1
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9
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6
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2
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728913
Yang
Purification, crystallization ...
Streptococcus pneumoniae
Acta Crystallogr. Sect. F
69
759-761
2013
-
-
1
1
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2
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695108
Gurha
Archaeal Pus10 proteins can pr ...
Methanocaldococcus jannaschii, Pyrococcus furiosus
RNA
14
2521-2527
2008
-
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2
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8
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705960
Muller
Identification of determinants ...
Pyrococcus abyssi
Nucleic Acids Res.
35
5610-5624
2007
1
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2
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1
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1
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706710
Gurha
Differential roles of archaeal ...
Methanocaldococcus jannaschii
RNA Biol.
4
101-109
2007
4
-
1
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5
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1
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705958
Roovers
Formation of the conserved pse ...
Pyrococcus furiosus
Nucleic Acids Res.
34
4293-4301
2006
1
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1
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1
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1
1
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-
661179
Phannachet
Dissecting the roles of a stri ...
Thermotoga maritima
Biochemistry
44
15488-15494
2005
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1
2
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1
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663343
Hoang
Precursor complex structure of ...
Escherichia coli
Protein Sci.
14
2201-2206
2005
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1
1
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652542
Chaudhuri
Crystal structure of the apo f ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
279
24585-24591
2004
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1
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706711
Spedaliere
Not all pseudouridine synthase ...
Escherichia coli
RNA
10
192-199
2004
-
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1
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2
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653587
Pan
Structure of tRNA pseudouridin ...
Escherichia coli, Thermotoga maritima
Proc. Natl. Acad. Sci. USA
100
12648-12653
2003
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2
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4
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2
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704264
Urbonavicius
Three modifications in the D a ...
Escherichia coli, Shigella flexneri
J. Bacteriol.
184
5348-5357
2002
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2
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705550
Kinghorn
Physiological analysis of the ...
Escherichia coli
Microbiology
148
3511-3520
2002
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1
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2
2
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650781
Hoang
Cocrystal structure of a tRNA ...
Escherichia coli
Cell
107
929-939
2001
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1
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702203
Spedaliere
Functional importance of motif ...
Escherichia coli
Biochemistry
39
9459-9465
2000
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5
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5
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1
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1
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1
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3
5
1
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5
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1
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5
1
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5
5
706733
Gutgsell
Deletion of the Escherichia co ...
Escherichia coli
RNA
6
1870-1881
2000
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648867
Ramamurthy
Role of cysteine residues in p ...
Escherichia coli
Biochemistry
38
13106-13111
1999
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1
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5
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5
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1
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1
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5
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1
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5
5
701450
Pienkowska
A cell-free yellow lupin extra ...
Lupinus luteus
Acta Biochim. Pol.
45
745-754
1998
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702196
Gu
Molecular recognition of tRNA ...
Escherichia coli
Biochemistry
37
339-343
1998
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2
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2
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1
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1
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1
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1
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1
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2
1
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648862
Becker
The yeast gene YNL292w encodes ...
Saccharomyces cerevisiae
Nucleic Acids Res.
25
4493-4499
1997
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1
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1
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706713
Nurse
Purification, cloning, and pro ...
Escherichia coli
RNA
1
102-112
1995
-
-
1
-
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2
-
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3
-
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1
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1
1
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1
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2
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1
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