Application | Comment | Organism |
---|---|---|
drug development | the tautomerase activity of MIF is a target for inhibitor development, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | modification of Pro1, e.g. via isothiocyanate inhibitors, alters the tertiary, but not the secondary or quaternary, structure of the trimer without affecting its thermodynamic stability, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-piperidinoethyl isothiocyanate | - |
Homo sapiens | |
allyl isothiocyamate | - |
Homo sapiens | |
Benzyl isothiocyanate | specific inhibition of MIF tautomerase activity is mediated by selective modification of the N-terminal proline | Homo sapiens | |
ethyl isothiocyanate | - |
Homo sapiens | |
methallyl isothiocyanate | - |
Homo sapiens | |
additional information | design and synthesis of irreversible isothiocyanate-based inhibitors of MIF, inhibitory potencies and inhibition mechanism, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | MIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant MIF from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3,4-dihydroxyphenylalanine methyl ester | the catalytic residue is N-terminal Pro1 | Homo sapiens | methyl 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)-L-alaninate | - |
r | |
additional information | MIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | trimer formation is required for MIF tautomerase activity, trimer three-dimensional structure and tautomerase active site structure. The subunit interface is not as hydrophobic as the interior of the monomer, however, there is a hydrophobic patch on the surface involving residues Y36, Y95, W108, and F113, MALDI-TOF and analytical gel filtration analysis, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
D-dopachrome tautomerase | - |
Homo sapiens |
Macrophage migration inhibitory factor | - |
Homo sapiens |
MIF | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0008 | - |
- |
Homo sapiens | Benzyl isothiocyanate | |
0.0014 | - |
- |
Homo sapiens | methallyl isothiocyanate | |
0.0031 | - |
- |
Homo sapiens | 2-piperidinoethyl isothiocyanate | |
0.0037 | - |
- |
Homo sapiens | allyl isothiocyamate | |
0.0111 | - |
- |
Homo sapiens | ethyl isothiocyanate |
General Information | Comment | Organism |
---|---|---|
malfunction | MIF is implicated in the pathogenesis of several inflammatory and autoimmune diseases | Homo sapiens |
physiological function | MIF is involved in diverse biological processes, e.g. MIF glucocorticoid overriding activity, endotoxin lipopolysaccharide-induced TNF production, MIF-mediated stimulation of ERK1/2 MAP kinase and proliferation of serum-starved cells, MIF-mediated upregulation of arachidonic acid in macrophages, and Cox-2 activation | Homo sapiens |