Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease | Saccharomyces cerevisiae |
40700 | - |
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease | Saccharomyces cerevisiae |
52500 | - |
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease | Saccharomyces cerevisiae |
100000 | - |
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor | Saccharomyces cerevisiae | - |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | Saccharomyces cerevisiae 20B-12-1 | - |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae 20B-12-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor | - |
Saccharomyces cerevisiae | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | - |
Saccharomyces cerevisiae 20B-12-1 | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease | Saccharomyces cerevisiae |