Cloned (Comment) | Organism |
---|---|
expression as a His-tagged fusion protein in Escherichia coli | Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-cysteine | pH 9.0, 37°C | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34500 | - |
x * 34500, calculated from sequence | Brassica napus |
35000 | - |
x * 35000, SDS-PAGE | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | J9WJN8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Brassica napus | - |
root | from seedling | Brassica napus | - |
seedling | root | Brassica napus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.066 | - |
pH 9.0, 37°C, purified enzyme | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | - |
Brassica napus | sulfide + NH3 + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Brassica napus |
? | x * 34500, calculated from sequence | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
At5g28030 | - |
Brassica napus |
DES1 | - |
Brassica napus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Brassica napus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | pH 8.0: about 30% of maximal activity, pH 9.5: about 65% of maximal activity | Brassica napus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brassica napus | assay at | - |
5.2 |
Organism | Comment | Expression |
---|---|---|
Brassica napus | BnDES1 expression is significantly down-regulated by cadmium | down |
Brassica napus | abscisic acid and gibberellic acid moderately induce the expression of the enzyme (BnDES1), and the maximal inducible time points are 24 h and 48 h, separately. Kinetin and indole-3-acetic acid induce the BnDES1 transcripts, with the same time point of 12 h for the maximal induction. BnDES1 transcripts are gradually up-regulated during 24 h or 12 h treatment periods by sodium nitroprusside or salicylic acid, and then fall back or keep the higher level until 48 h, respectively. The addition of paraquat brings about the rapid and moderated induction of BnDES1 during 3 h treatment period. BnDES1 transcript is induced sharply by the NaHS and L-cysteine treatments, both with maximum induction at 12 h following treatments | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is responsible for the H2S production, and may play an important role in plant growth regulators and chemical stimuli responses | Brassica napus |