BRENDA - Enzyme Database show
show all sequences of 4.3.3.6

Defining the structural requirements for ribose 5-phosphate-binding and intersubunit cross-talk of the malarial pyridoxal 5-phosphate synthase

Derrer, B.; Windeisen, V.; Guedez Rodriguez, G.; Seidler, J.; Gengenbacher, M.; Lehmann, W.; Rippe, K.; Sinning, I.; Tews, I.; Kappes, B.; FEBS Lett. 584, 4169-4174 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL
Plasmodium falciparum
Engineering
Amino acid exchange
Commentary
Organism
DELTA 270-301
mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 273-301
dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 279-301
dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 287-301
dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate
Plasmodium falciparum
DELTA 293-301
behaviour like DELTA 287-301
Plasmodium falciparum
DELTA 295-301
behaviour like DELTA 287-301
Plasmodium falciparum
S270A/DELTA 273-301
dodecamer
Plasmodium falciparum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS
Plasmodium falciparum
363000
-
analytical ultracentrifugation, 420000 calculated
Plasmodium falciparum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Plasmodium falciparum
C6KT50
-
-
Purification (Commentary)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration
Plasmodium falciparum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00025
-
DELTA 279-301 protein, in the presence of Pdx2, pH 8.0, 37°C
Plasmodium falciparum
0.000779
-
wild-type protein, in the presence of Pdx2, pH 8.0, 37°C
Plasmodium falciparum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine
in the presence of Pdx2
724999
Plasmodium falciparum
pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
-
-
?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
in the absence of Pdx2
724999
Plasmodium falciparum
pyridoxal 5'-phosphate + 4 H2O + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dodecamer
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS
Plasmodium falciparum
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL
Plasmodium falciparum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
DELTA 270-301
mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 273-301
dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 279-301
dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis
Plasmodium falciparum
DELTA 287-301
dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate
Plasmodium falciparum
DELTA 293-301
behaviour like DELTA 287-301
Plasmodium falciparum
DELTA 295-301
behaviour like DELTA 287-301
Plasmodium falciparum
S270A/DELTA 273-301
dodecamer
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS
Plasmodium falciparum
363000
-
analytical ultracentrifugation, 420000 calculated
Plasmodium falciparum
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration
Plasmodium falciparum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00025
-
DELTA 279-301 protein, in the presence of Pdx2, pH 8.0, 37°C
Plasmodium falciparum
0.000779
-
wild-type protein, in the presence of Pdx2, pH 8.0, 37°C
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine
in the presence of Pdx2
724999
Plasmodium falciparum
pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
-
-
?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
in the absence of Pdx2
724999
Plasmodium falciparum
pyridoxal 5'-phosphate + 4 H2O + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dodecamer
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS
Plasmodium falciparum
Other publictions for EC 4.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748203
Smith
Crystal structures capture th ...
Geobacillus kaustophilus
J. Biol. Chem.
290
5226-5239
2015
-
-
1
1
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-
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3
-
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1
-
2
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1
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3
1
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3
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1
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1
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3
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1
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1
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-
3
1
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3
-
-
-
-
-
-
-
-
-
-
730418
Belitsky
Role of PdxR in the activation ...
Listeria monocytogenes
Mol. Microbiol.
92
1113-1128
2014
1
-
1
-
1
-
-
-
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1
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1
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1
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1
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-
-
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-
-
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-
-
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-
724239
Reeksting
Exploring inhibition of Pdx1, ...
Plasmodium falciparum
Biochem. J.
449
175-187
2013
-
-
1
-
5
-
5
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
5
-
5
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
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-
-
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-
-
-
-
-
729134
Kim
Crystal structure of Mycobacte ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochem. Biophys. Res. Commun.
435
255-259
2013
-
1
1
1
1
-
-
-
-
1
-
-
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3
-
-
1
-
-
-
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1
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1
1
-
1
1
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1
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-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
729433
Itagaki
Differences in the roles of a ...
Bacillus circulans, Bacillus circulans SANK 72073, Bacillus subtilis
Biosci. Biotechnol. Biochem.
77
1481-1485
2013
-
-
2
-
1
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721233
Yoon
Overexpression, crystallizatio ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Acta Crystallogr. Sect. F
68
440-442
2012
-
-
1
1
-
-
-
-
-
-
2
-
-
9
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723192
Matsuura
Crystal structure of pyridoxal ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Mol. Cells
34
407-412
2012
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
1
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-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726499
Guedez
Assembly of the eukaryotic PLP ...
Plasmodium berghei, Plasmodium falciparum
Structure
20
172-184
2012
-
-
2
2
8
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
28
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
8
-
-
-
-
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-
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-
-
-
2
-
-
28
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716150
Jochmann
Positive transcriptional contr ...
Corynebacterium glutamicum, Corynebacterium glutamicum NJ0898
Microbiology
157
77-88
2011
-
-
-
-
-
-
-
-
-
-
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2
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-
-
-
-
-
-
-
-
2
1
1
2
-
-
726269
Moccand
It takes two to Tango: Definin ...
Bacillus subtilis
PLoS ONE
6
e16042
2011
-
-
1
-
8
-
-
24
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
13
-
-
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-
-
-
-
-
-
1
-
-
8
-
-
-
-
24
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
-
-
-
25
25
724219
Zhang
Structural insights into the c ...
Saccharomyces cerevisiae
Biochem. J.
432
445-450
2010
-
-
1
1
6
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
1
-
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-
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1
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1
6
-
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1
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-
1
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-
-
-
-
-
-
-
-
-
-
-
724999
Derrer
Defining the structural requir ...
Plasmodium falciparum
FEBS Lett.
584
4169-4174
2010
-
-
1
-
7
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
2
-
2
1
-
-
-
-
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-
-
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-
1
-
-
7
-
-
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-
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-
-
2
-
-
-
-
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714170
Wallner
Dissection of contributions fr ...
Bacillus subtilis
Biochemistry
48
1928-1935
2009
-
-
1
-
10
-
-
-
-
-
-
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-
2
-
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-
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-
1
-
1
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1
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10
-
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1
-
1
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-
-
-
-
-
713758
Hanes
Trapping of a chromophoric int ...
Bacillus subtilis
Angew. Chem. Int. Ed. Engl.
47
2102-2105
2008
-
-
-
-
-
-
-
-
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1
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1
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1
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-
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-
716306
Hanes
13C NMR snapshots of the compl ...
Bacillus subtilis
Nat. Chem. Biol.
4
425-430
2008
-
-
-
-
-
-
-
-
-
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1
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1
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2
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1
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2
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715491
Raschle
Reaction mechanism of pyridoxa ...
Bacillus subtilis
J. Biol. Chem.
282
6098-6105
2007
-
-
-
-
5
-
-
2
-
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3
-
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2
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1
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4
1
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5
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2
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2
-
1
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4
1
-
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-
-
2
2
716736
Strohmeier
Structure of a bacterial pyrid ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
103
19284-19289
2006
-
-
1
1
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-
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5
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1
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1
1
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1
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1
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1
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1
1
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-
715247
Burns
Reconstitution and biochemical ...
Bacillus subtilis
J. Am. Chem. Soc.
127
3682-3683
2005
-
-
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1
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1
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1
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1
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1
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-
715478
Raschle
On the two components of pyrid ...
Bacillus subtilis
J. Biol. Chem.
280
32291-32300
2005
-
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1
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1
3
-
1
-
1
-
1
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1
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4
-
1
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2
1
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1
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-
-
-
1
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3
-
1
-
1
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-
-
1
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-
-
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4
-
1
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2
1
-
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-
-
-
-
-
2
2