Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method, 16°C | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E116A | activity as the wild-type protein | Saccharomyces cerevisiae |
K117A | no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization | Saccharomyces cerevisiae |
K148A | no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization | Saccharomyces cerevisiae |
K240A | activity as the wild-type protein | Saccharomyces cerevisiae |
R136A/R137A | no synthesis of pyridoxal 5'-phosphate, dihydroxyacetone phosphate isomerization activity as the wild-type protein | Saccharomyces cerevisiae |
R164A | completely inactive, dihydroxyacetone phosphate isomerization activity as the wild-type protein | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q03148 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribulose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyridoxal 5-phosphate synthase Snz1 | - |
Saccharomyces cerevisiae |
Snz1 | - |
Saccharomyces cerevisiae |