BRENDA - Enzyme Database show
show all sequences of 4.3.2.6

Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain

Llewellyn, N.M.; Li, Y.; Spencer, J.B.; Chem. Biol. 14, 379-386 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus circulans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18100
-
1 * 18100, calculated from amino acid sequence
Bacillus circulans
20093
-
1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
Bacillus circulans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus circulans
-
-
-
Bacillus circulans NR3312
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-NTA affinity column chromatography
Bacillus circulans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gamma-L-glutamyl-butirosin B
-
719271
Bacillus circulans
butirosin B + 5-oxoproline
-
-
-
?
gamma-L-glutamyl-butirosin B
-
719271
Bacillus circulans NR3312
butirosin B + 5-oxoproline
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 18100, calculated from amino acid sequence; 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
Bacillus circulans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus circulans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18100
-
1 * 18100, calculated from amino acid sequence
Bacillus circulans
20093
-
1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
Bacillus circulans
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA affinity column chromatography
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gamma-L-glutamyl-butirosin B
-
719271
Bacillus circulans
butirosin B + 5-oxoproline
-
-
-
?
gamma-L-glutamyl-butirosin B
-
719271
Bacillus circulans NR3312
butirosin B + 5-oxoproline
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 18100, calculated from amino acid sequence; 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
Bacillus circulans
General Information
General Information
Commentary
Organism
metabolism
the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin
Bacillus circulans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin
Bacillus circulans
Other publictions for EC 4.3.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718529
Nudelman
-
Combined chemical-enzymatic as ...
Bacillus circulans
Adv. Synth. Catal.
350
1682-1688
2008
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719271
Llewellyn
Biosynthesis of butirosin: tra ...
Bacillus circulans, Bacillus circulans NR3312
Chem. Biol.
14
379-386
2007
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