Cloned (Comment) | Organism |
---|---|
gene Bc3750, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | 77% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
UDP-galactose | 51% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
UDP-GalNAc | 91% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
UDP-glucose | 81% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
UTP | 98% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
recombinant His6-tagged enzyme, gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q81A42 | - |
- |
Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | Q81A42 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg | Bacillus cereus | ? | - |
? | |
additional information | product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg | Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | ? | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, recombinant His6-tagged enzyme, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
Bc3750 | - |
Bacillus cereus |
BC_3750 | - |
Bacillus cereus |
Pdeg | - |
Bacillus cereus |
UDP-Glc-NAc 4,6-dehydratase | - |
Bacillus cereus |
UDP-N-acetylglucosamine C4,6-dehydratase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | recombinant His6-tagged enzyme | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified recombinant His6-tagged enzyme, 2 min, inactivation | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant His6-tagged enzyme | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzme is involved in the biosynthesis of Bacillus glycans and N-acetylquinovosamine (2-acetamido-2,6-di-deoxy-D-glucose, QuiNAc) | Bacillus cereus |
physiological function | gene Pdeg encodes an UDP-N-acetylglucosamine C4,6-dehydratase that converts UDP-GlcNAc to UDP-4-keto-4,6-D-deoxy-GlcNAc (UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose). The product is then catalyzed by Preq-encoded UDP-4-reductase that converts UDP-4-keto-4,6-d-deoxy-GlcNAc to UDP-N-acetylquinovosamine | Bacillus cereus |