Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | anaerobic reconstitution of apoenzyme with Fe2+, which yields an enzyme with an effective kcat that exceeds that for the Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful | Methanosarcina thermophila | |
Fe2+ | the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful | Methanosarcina thermophila | |
additional information | reconstitution of the apoenzyme with Cu2+, Mn2+, Ni2+, or Cd2+ yields enzymes with effective kcat values that are 10% or less than the value for the Zn2+-reconstituted apoenzyme | Methanosarcina thermophila | |
Zn2+ | the enzyme contains contains catalytic zinc | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Methanosarcina thermophila | P40881 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant enzyme is purified anaerobically | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Methanosarcina thermophila | H2CO3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cam | - |
Methanosarcina thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ | Methanosarcina thermophila | |
additional information | - |
additional information | the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme | Methanosarcina thermophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ | Methanosarcina thermophila | |
additional information | - |
additional information | the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme | Methanosarcina thermophila |