Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells, HPS only, or as fusion enzyme with 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.96 | - |
formaldehyde | purified recombinant enzyme, at 30°C | Mycobacterium gastri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Mycobacterium gastri |
45000 | - |
gel filtration | Mycobacterium gastri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium gastri | - |
- |
- |
Mycobacterium gastri MB19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography butyl-Toyopearl column chromatography | Mycobacterium gastri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.1 | - |
cell free extract, recombinant HPS | Mycobacterium gastri |
70.9 | - |
after 1.73fold purification, recombinant HPS | Mycobacterium gastri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri MB19 | D-arabino-hex-3-ulose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22000, SDS-PAGE | Mycobacterium gastri |
Synonyms | Comment | Organism |
---|---|---|
3-hexulose-6-phosphate synthase | - |
Mycobacterium gastri |
HPS | - |
Mycobacterium gastri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme | Mycobacterium gastri |