Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,4-Trihydroxybenzoate | complete inhibition of carboxylation of phenol | Desulfovibrio sp. | |
2,3,4-Trihydroxybenzoate | complete inhibition of carboxylation of phenol | Methanospirillum hungatei | |
2,4,6-Trihydroxybenzoate | complete inhibition of carboxylation of phenol | Desulfovibrio sp. | |
2,4,6-Trihydroxybenzoate | complete inhibition of carboxylation of phenol | Methanospirillum hungatei | |
2,4-Dihydroxybenzoate | - |
Desulfovibrio sp. | |
2,4-Dihydroxybenzoate | - |
Methanospirillum hungatei | |
3,4,5-Trihydroxybenzoate | slight inhibition of carboxylation of phenol | Desulfovibrio sp. | |
3,4,5-Trihydroxybenzoate | slight inhibition of carboxylation of phenol | Methanospirillum hungatei | |
3-Chloro-4-hydroxybenzoate | - |
Desulfovibrio sp. | |
3-Chloro-4-hydroxybenzoate | - |
Methanospirillum hungatei | |
Co2+ | - |
Desulfovibrio sp. | |
Co2+ | - |
Methanospirillum hungatei | |
EDTA | - |
Desulfovibrio sp. | |
EDTA | - |
Methanospirillum hungatei | |
m-Hydroxybenzoylhydrazine dichloride | - |
Desulfovibrio sp. | |
m-Hydroxybenzoylhydrazine dichloride | - |
Methanospirillum hungatei | |
Sodium dithionite | - |
Desulfovibrio sp. | |
Sodium dithionite | - |
Methanospirillum hungatei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
4-hydroxybenzoate | carboxylase with pH-optimum 7.8 | Methanospirillum hungatei | |
0.004 | - |
4-hydroxybenzoate | carboxylase with pH-optimum 7.8 | Desulfovibrio sp. | |
0.02 | - |
4-hydroxybenzoate | carboxylase with pH-optimum 6.4 | Methanospirillum hungatei | |
0.02 | - |
4-hydroxybenzoate | carboxylase with pH-optimum 6.4 | Desulfovibrio sp. | |
0.125 | - |
phenol | - |
Methanospirillum hungatei | |
0.125 | - |
phenol | - |
Desulfovibrio sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | loosely associated | Methanospirillum hungatei | 16020 | - |
membrane | loosely associated | Desulfovibrio sp. | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on | Methanospirillum hungatei | |
K+ | phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on | Desulfovibrio sp. | |
Mn2+ | restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 | Methanospirillum hungatei | |
Mn2+ | restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 | Desulfovibrio sp. | |
Na+ | stimulates activity of the enzyme form with pH-optimum pH 7.8 | Methanospirillum hungatei | |
Na+ | stimulates activity of the enzyme form with pH-optimum pH 7.8 | Desulfovibrio sp. | |
NH4+ | stimulates activity of the enzyme form with pH-optimum pH 7.8 | Methanospirillum hungatei | |
NH4+ | stimulates activity of the enzyme form with pH-optimum pH 7.8 | Desulfovibrio sp. | |
Rb+ | can replace K+ in the phenol carboxylase and partially in both decarboxylases | Methanospirillum hungatei | |
Rb+ | can replace K+ in the phenol carboxylase and partially in both decarboxylases | Desulfovibrio sp. | |
Zn2+ | compensates the loss of activity by EDTA | Methanospirillum hungatei | |
Zn2+ | compensates the loss of activity by EDTA | Desulfovibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio sp. | - |
in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties | - |
Methanospirillum hungatei | - |
in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties | - |
Storage Stability | Organism |
---|---|
-20°C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months | Methanospirillum hungatei |
-20°C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months | Desulfovibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4,5-Trihydroxybenzoate | - |
Methanospirillum hungatei | Pyrogallol + CO2 | - |
? | |
3,4,5-Trihydroxybenzoate | - |
Desulfovibrio sp. | Pyrogallol + CO2 | - |
? | |
3,5-Dihydroxybenzoate | - |
Methanospirillum hungatei | 1,3-Dihydroxybenzene + CO2 | - |
? | |
3,5-Dihydroxybenzoate | - |
Desulfovibrio sp. | 1,3-Dihydroxybenzene + CO2 | - |
? | |
3,5-Dimethoxy-4-hydroxybenzoate | - |
Methanospirillum hungatei | 1,3-Dimethoxy-2-hydroxybenzene + CO2 | - |
? | |
3,5-Dimethoxy-4-hydroxybenzoate | - |
Desulfovibrio sp. | 1,3-Dimethoxy-2-hydroxybenzene + CO2 | - |
? | |
3-Chloro-4-hydroxybenzoate | - |
Methanospirillum hungatei | 1-Chloro-2-hydroxybenzene + CO2 | - |
? | |
3-Chloro-4-hydroxybenzoate | - |
Desulfovibrio sp. | 1-Chloro-2-hydroxybenzene + CO2 | - |
? | |
4-Hydroxybenzoate | - |
Methanospirillum hungatei | Phenol + CO2 | - |
? | |
4-Hydroxybenzoate | - |
Desulfovibrio sp. | Phenol + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme with pH-optimum of pH 7.8 | Methanospirillum hungatei |
37 | - |
enzyme with pH-optimum of pH 7.8 | Desulfovibrio sp. |
41 | - |
enzyme with pH-optimum of pH 6.4 | Methanospirillum hungatei |
41 | - |
enzyme with pH-optimum of pH 6.4 | Desulfovibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Methanospirillum hungatei |
6.4 | - |
- |
Desulfovibrio sp. |
6.5 | - |
phenol carboxylation | Methanospirillum hungatei |
6.5 | - |
phenol carboxylation | Desulfovibrio sp. |
7.8 | - |
- |
Methanospirillum hungatei |
7.8 | - |
- |
Desulfovibrio sp. |