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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
4-hydroxybenzoate decarboxylase, phenol carboxylase,
more
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4-hydroxybenzoate decarboxylase
-
Decarboxylase, p-hydroxybenzoate
-
-
-
-
p-Hydroxybenzoate decarboxylase
-
-
-
-
Phenol carboxylase
-
-
-
-
vanillate/4-hydroxybenzoate decarboxylase
-
HBDC
-
-
KpdB
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxybenzoate = phenol + CO2
4-hydroxybenzoate = phenol + CO2
mechanism
-
4-hydroxybenzoate = phenol + CO2
biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction
-
4-hydroxybenzoate = phenol + CO2
biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction
-
-
4-hydroxybenzoate = phenol + CO2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
decarboxylation
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxybenzoate carboxy-lyase (phenol-forming)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
3,4-Dihydroxybenzoate
Catechol + CO2
-
-
-
?
3,5-Dihydroxybenzoate
1,3-Dihydroxybenzene + CO2
3,5-Dimethoxy-4-hydroxybenzoate
1,3-Dimethoxy-2-hydroxybenzene + CO2
3-Chloro-4-hydroxybenzoate
1-Chloro-2-hydroxybenzene + CO2
3-methoxy-4-hydroxybenzoate
2-methoxyphenol + CO2
vanillate
guaiacol
-
?
4-Hydroxybenzoate
Phenol + CO2
Catechol + CO2
3,4-Dihydroxybenzoate
-
-
-
?
Phenol + CO2
4-Hydroxybenzoate
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
additional information
?
-
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
-
-
-
-
?
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
-
-
-
-
?
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
-
activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate
-
-
?
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
-
activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate
-
-
?
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
-
slow reaction
-
?
3,5-Dihydroxybenzoate
1,3-Dihydroxybenzene + CO2
-
-
-
-
?
3,5-Dihydroxybenzoate
1,3-Dihydroxybenzene + CO2
-
-
-
-
?
3,5-Dimethoxy-4-hydroxybenzoate
1,3-Dimethoxy-2-hydroxybenzene + CO2
-
-
-
-
?
3,5-Dimethoxy-4-hydroxybenzoate
1,3-Dimethoxy-2-hydroxybenzene + CO2
-
-
-
-
?
3-Chloro-4-hydroxybenzoate
1-Chloro-2-hydroxybenzene + CO2
-
-
-
-
?
3-Chloro-4-hydroxybenzoate
1-Chloro-2-hydroxybenzene + CO2
-
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
-
-
-
?
Phenol + CO2
4-Hydroxybenzoate
-
weak reaction
-
-
r
Phenol + CO2
4-Hydroxybenzoate
-
weak reaction
-
-
r
Phenol + CO2
4-Hydroxybenzoate
-
carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19%
-
-
?
Phenol + CO2
4-Hydroxybenzoate
-
carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19%
-
-
?
Phenol + CO2
4-Hydroxybenzoate
-
-
-
-
?
Phenol + CO2
4-Hydroxybenzoate
-
-
-
-
?
Phenol + CO2
4-Hydroxybenzoate
-
-
-
?
Phenol + CO2
?
-
first step in the anaerobic metabolism of phenol
-
-
?
Phenol + CO2
?
-
first step in the anaerobic metabolism of phenol
-
-
?
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
-
-
-
?
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
-
-
-
-
?
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
-
-
-
?
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
-
-
-
-
?
additional information
?
-
no reaction with: 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,6-dihydroxybenzoate, and 3,4-dihydroxybenzoate, 2,3,4-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, 3,5-dihydroxy-2-naphthalene dicarboxylate, 2,8-dihydroxynaphthoate, 5-hydroxyisophthalate, 1-hydroxy-2-naphthoate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, 6-hydroxynicotinate, 2-hydroxypyridine-3-carboxylate, 3-hydroxypyridine-2-carboxylate
-
-
?
additional information
?
-
-
no reaction with: 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,6-dihydroxybenzoate, and 3,4-dihydroxybenzoate, 2,3,4-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, 3,5-dihydroxy-2-naphthalene dicarboxylate, 2,8-dihydroxynaphthoate, 5-hydroxyisophthalate, 1-hydroxy-2-naphthoate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, 6-hydroxynicotinate, 2-hydroxypyridine-3-carboxylate, 3-hydroxypyridine-2-carboxylate
-
-
?
additional information
?
-
-
benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates
-
-
?
additional information
?
-
-
benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates
-
-
?
additional information
?
-
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induced by 4-hydroxybenzoate
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-
?
additional information
?
-
-
catalyzes a CO2:4-hydroxybenzoate isotope exchange reaction
-
-
?
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
?
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
?
additional information
?
-
-
catalyzes a CO2:4-hydroxybenzoate isotope exchange reaction
-
-
?
additional information
?
-
-
no substrates are 2-hydroxybenzoate, 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,3,4-trihydroxybenzoate, 3,4,5-trihydroxybenzoate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
?
-
Phenol + CO2
?
-
first step in the anaerobic metabolism of phenol
-
-
?
Phenol + CO2
?
-
first step in the anaerobic metabolism of phenol
-
-
?
additional information
?
-
-
induced by 4-hydroxybenzoate
-
-
?
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
?
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
not dependent on biotin, ATP, thiamin or pyridoxal phosphate
-
additional information
-
not dependent on biotin, ATP, thiamin or pyridoxal phosphate
-
additional information
-
the activity of 4-hydroxybenzoate decarboxylase is independent of pyridoxal 5'-phosphate, thiamine diphosphate, and the pyruvoyl group
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
-
1 mM, 1.44fold stimulation of activity
CaCl2
-
2.5 mM, 99% stimulation of activity
CdCl2
-
2.5 mM, 51% stimulation of activity
Co2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
MgSO4
-
2.5 mM, 125% stimulation of activity
MnCl2
-
2.5 mM, 112% stimulation of activity
ZnCl2
-
2.5 mM, 75% stimulation of activity
ZnSO4
-
2.5 mM, 100% stimulation of activity
Fe2+
-
1 mM, 2.52fold stimulation of activity
Fe2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
K+
-
phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on
K+
-
phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on
K+
-
required for CO2:4-hydroxybenzoate isotope exchange reaction
K+
-
not required for phenylphosphate decarboxylase activity
Mg2+
-
1 mM, 2.99fold stimulation of activity
Mg2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Mn2+
-
1 mM, 2.43fold stimulation of activity
Mn2+
-
restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8
Mn2+
-
restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8
Mn2+
-
required for CO2:4-hydroxybenzoate isotope exchange reaction
Mn2+
-
required for phenylphosphate decarboxylase activity
Mn2+
-
minor stimulation of carboxylation of 4-hydroxybenzoate
Na+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
Na+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
NH4+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
NH4+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
Rb+
-
can replace K+ in the phenol carboxylase and partially in both decarboxylases
Rb+
-
can replace K+ in the phenol carboxylase and partially in both decarboxylases
Rb+
-
can replace to some extent for K+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Zn2+
-
compensates the loss of activity by EDTA
Zn2+
-
compensates the loss of activity by EDTA
additional information
-
not stimulated by Cu2+ or Zn2+
additional information
-
AgNO3, FeCl2, CoCl2, NiCl2, and FeSO4 do not affect activity
additional information
no metal ions
additional information
-
no metal ions
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3-Chloro-4-hydroxybenzoate
CuCl2
-
2.5 mM, 91% loss of activity
CuSO4
-
2.5 mM, 92% loss of activity
HgCl2
-
2.5 mM, 78% loss of activity
m-Hydroxybenzoylhydrazine dichloride
NH4+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Rb+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Zn2+
-
destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate
additional information
-
not significantly affected by phenylhydrazine or hydroxylamine
-
2,3,4-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,3,4-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,3,4-Trihydroxybenzoate
-
-
2,4,6-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4,6-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4,6-Trihydroxybenzoate
-
-
2,4-Dihydroxybenzoate
-
-
2,4-Dihydroxybenzoate
-
-
2,4-Dihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
slight inhibition of carboxylation of phenol
3,4,5-Trihydroxybenzoate
-
slight inhibition of carboxylation of phenol
3,4,5-Trihydroxybenzoate
-
-
3-Chloro-4-hydroxybenzoate
-
-
3-Chloro-4-hydroxybenzoate
-
-
Co2+
-
-
EDTA
-
-
EDTA
-
2.5 mM, 22% inhibition
EDTA
-
47% inhibition at 5 mM, Mg2+, Mn2+, Zn2+ or Ca2+ protect, Fe2+ does not
m-Hydroxybenzoylhydrazine dichloride
-
-
m-Hydroxybenzoylhydrazine dichloride
-
-
Sodium dithionite
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
1 mM pyridoxal 5'-phosphate or thiamine diphosphate do not enhance activity
-
additional information
-
no stimulation by ATP up to 5 mM or phenylphosphate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.2 - 6.8
3,4-dihydroxybenzoate
0.004 - 5.4
4-hydroxybenzoate
0.2
Phenylphosphate
-
CO2:4-hydroxybenzoate isotope exchange reaction
additional information
additional information
-
1.2
3,4-dihydroxybenzoate
-
-
6.8
3,4-dihydroxybenzoate
-
in 100 mM potassium phosphate buffer (pH 7.0)
0.004
4-hydroxybenzoate
-
carboxylase with pH-optimum 7.8
0.004
4-hydroxybenzoate
-
carboxylase with pH-optimum 7.8
0.02
4-hydroxybenzoate
-
carboxylase with pH-optimum 6.4
0.02
4-hydroxybenzoate
-
carboxylase with pH-optimum 6.4
0.21
4-hydroxybenzoate
-
in 20 mM Tris-HCl (pH 7.5) containing 1 mM Mg2+, at 30°C
0.4
4-hydroxybenzoate
-
-
0.596
4-hydroxybenzoate
-
in 100 mM potassium phosphate buffer (pH 7.0)
0.67
4-hydroxybenzoate
-
-
5.4
4-hydroxybenzoate
-
pH 6.0, 37°C
1
CO2
-
dissolved, CO2:4-hydroxybenzoate isotope exchange reaction
0.125
phenol
-
-
14.8
phenol
-
20 mM phenol, 3 MKHCO3, 10 mM dithiothreitol in 100 mM potassium phosphate buffer (pH 7.0)
additional information
additional information
4-hydroxybenzoate as substrate Km = 1 mmol/l
-
additional information
additional information
-
4-hydroxybenzoate as substrate Km = 1 mmol/l
-
additional information
additional information
vanillate as substrate Km = 4 mmol/l
-
additional information
additional information
-
vanillate as substrate Km = 4 mmol/l
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
18.3
3,4-dihydroxybenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00025
-
crude cell extract, at 30°C
0.00506
-
after purification, at 30°C
0.012
-
phenylphosphate carboxylation
11
BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 3-methoxy-4-hydroxybenzoate as substrate
13.7
-
at 30°C, pH 7.0, crude cell extract
14
BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 4-hydroxybenzoate as substrate
15
uninduced, 3-methoxy-4-hydroxybenzoate as substrate
16
BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 3-methoxy-4-hydroxybenzoate as substrate
17.8
-
at 30°C, pH 7.0, after 1.5fold purification
2.5
uninduced, 4-hydroxybenzoate as substrate
23
BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 4-hydroxybenzoate as substrate
25
3-methoxy-4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate
30
4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate
31
4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate
6
3-methoxy-4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate
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6.4
-
-
6.5
-
phenol carboxylation
6.5
-
in potassium phosphate buffer
6.5
-
phenol carboxylation
6.5
-
phenylphosphate carboxylation
7.8
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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30
-
-
30
-
in potassium phosphate buffer
37
-
enzyme with pH-optimum of pH 7.8
37
-
enzyme with pH-optimum of pH 7.8
41
-
enzyme with pH-optimum of pH 6.4
41
-
enzyme with pH-optimum of pH 6.4
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.6 - 5.9
-
isoeolctric focusing
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ATCC 6051
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
brenda
-
-
-
brenda
-
-
-
brenda
AFH12530 i.e. subunit YclB, AFH12529 i.e. subunit YclC, AFH12528 i.e. subunit YclD
AFH12530; AFH12529; AFH12528
GenBank
brenda
AFH12530 i.e. subunit YclB, AFH12529 i.e. subunit YclC, AFH12528 i.e. subunit YclD
AFH12530; AFH12529; AFH12528
GenBank
brenda
Aerobacter aerogenes
-
-
brenda
B-subunit
UniProt
brenda
B-subunit
UniProt
brenda
in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Clostridium-like strain 6
-
-
brenda
-
UniProt
brenda
Clostridium hydroxybenzoicum, strain JW/Z-1T
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
-
induced in growing and resting cells by 4-hydroxybenzoate
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
loosely associated
brenda
-
loosely associated
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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315800
-
calculated from amino acid sequence
350000
-
gradient PAGE, gel filtration
372000
-
gel-filtration HPLC
57000
-
6 * 57000, SDS-PAGE
60000
-
6 * 60000, X-ray crystallography
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hexamer
-
6 * 57000, SDS-PAGE
homohexamer
-
6 * 60000, X-ray crystallography
homohexamer
-
6 * 60000, X-ray crystallography
-
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5.5 - 7.3
-
stable for 2 h
4740
6
-
11-40°C, stable for more than 24 h
4740
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11 - 40
-
pH 6.0, stable for more than 24 h
20
-
when the enzyme is incubated at temperatures up to 20°C for 30 min, activity is barely decreased
30 - 60
-
treatment at 30, 40, 50, and 60°C causes 32, 43, 96, and 100% losses of the initial activity, respectively
40 - 50
-
loses more than 50% decarboxylase activity at 40°C for 5 min, and less than 25% enzyme activity remains after incubation at 50°C for 5 min
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extremely sensitive to oxygen, in contact with air, loss of activity within a few hours
-
oxygen sensitive, loss of 50% of activity after 1 h in presence of air at 4°C compared to no activity lost after 7 days under anaerobic conditions at 4°C
-
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half-life in air-saturated solution: less than 1 min
-
4742
oxygen-sensitive, 50% loss of activity when exposed to air at 5°C for 2 h
-
4740
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-20C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months
-20C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months
-
-20C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months
-
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DEAE-Sephacel column chromatography, phenyl-Sepharose CL-4B column chromatography, and butyl-Toyopearl 650M column chromatography
-
under anaerobic conditions (oxygen-free N2) at 4°C by affinity chromatography
-
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expressed in Escherichia coli
expressed in Escherichia coli strain BL21 (DE3)
-
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biotechnology
-
use of enzyme for conversion of phenol into 4-hydroxybenzoic acid
synthesis
AFH12530; AFH12529; AFH12528
construction of a phenol synthetic pathway in Escherichia coli via overexpression of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and chorismate pyruvate lyase. Phenol titer increases 147-fold after modulating the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, chorismate pyruvate lyase, and 4-hydroxybenzoate decarboxylase genes in the chromosome. Tributyrin and dibutyl phthalate are the best two solvents for improving phenol production
synthesis
increase in protocatechuate decarboxylase activity in Escherichia coli through enhanced expression of both protocatechuate decarboxylase AroYand kpdB encoding the B-subunit of 4-hydroxybenzoate decarboxylase. This permits expression of protocatechuate decarboxylase activity at a level approximately 14fold greater than the strain with aroY only. The expression level of AroY increases, apparently as a function of the coexpression of AroY and KpdB
synthesis
-
increase in protocatechuate decarboxylase activity in Escherichia coli through enhanced expression of both protocatechuate decarboxylase AroYand kpdB encoding the B-subunit of 4-hydroxybenzoate decarboxylase. This permits expression of protocatechuate decarboxylase activity at a level approximately 14fold greater than the strain with aroY only. The expression level of AroY increases, apparently as a function of the coexpression of AroY and KpdB
-
synthesis
-
construction of a phenol synthetic pathway in Escherichia coli via overexpression of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and chorismate pyruvate lyase. Phenol titer increases 147-fold after modulating the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, chorismate pyruvate lyase, and 4-hydroxybenzoate decarboxylase genes in the chromosome. Tributyrin and dibutyl phthalate are the best two solvents for improving phenol production
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Grant, D.J.W.; Patel, J.C.
The non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid and gallic acid by Klebsiella aerogenes (Aerobacter aerogenes)
Antonie van Leeuwenhoek
35
325-343
1969
Klebsiella aerogenes
brenda
He, Z.; Wiegel, J.
Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum
Eur. J. Biochem.
229
77-82
1995
Sedimentibacter hydroxybenzoicus
brenda
Gallert, C.; Winter, J.
Comparison of 4-hydroxybenzoate decarboxylase, and phenol carboxylase activities in cell-free extracts of a defined 4-hydroxybenzoate and phenol-degrading anaerobic consortium
Appl. Microbiol. Biotechnol.
37
119-124
1992
Desulfovibrio sp., Methanospirillum hungatei
-
brenda
Lack, A.; Fuchs, G.
Carboxylation of phenylphosphate by phenol carboxylase, an enzyme system of anaerobic phenol metabolism
J. Bacteriol.
174
3629-3636
1992
Pseudomonas sp., Pseudomonas sp. K172
brenda
Hsu, T.; Lux, M.F.; Drake, H.L.
Expression of an aromatic-dependent decarboxylase which provides growth-essential CO2 equivalents for the acetogenic (wood) pathway of Clostridium thermoaceticum
J. Bacteriol.
172
5901-5907
1990
Moorella thermoacetica
brenda
Lack, A.; Tommasi, I.; Aresta, M.; Fuchs, G.
Catalytic properties of phenol carboxylase. In vitro study of CO2:4hydroxybenzoate isotope exchange reaction
Eur. J. Biochem.
197
473-479
1991
Pseudomonas sp., Pseudomonas sp. K172
brenda
Li, T.; Juteau, P.; Beaudet, R.; Lepine, F.; Villemur, R.; Bisaillon, J.G.
Purification and characterization of a 4-hydroxybenzoate decarboxylase from an anaerobic coculture
Can. J. Microbiol.
46
856-859
2000
uncultured bacterium
brenda
Huang, J.; He, Z.; Wiegel, J.
Cloning, characterization, and expression of a novel gene encoding a reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum
J. Bacteriol.
181
5119-5122
1999
Sedimentibacter hydroxybenzoicus (Q4R101 and Q9S4M7 and Q4R102), Sedimentibacter hydroxybenzoicus
brenda
Aresta, M.; Dibenedetto, A.
Development of environmentally friendly syntheses: use of enzymes and biomimetic systems for the direct carboxylation of organic substrates
J. Biotechnol.
90
113-128
2002
Thauera aromatica
brenda
Matsui, T.; Yoshida, T.; Hayashi, T.; Nagasawa, T.
Purification, characterization, and gene cloning of 4-hydroxybenzoate decarboxylase of Enterobacter cloacae P240
Arch. Microbiol.
186
21-29
2006
Enterobacter cloacae, Enterobacter cloacae P240
brenda
Liu, J.; Zhang, X.; Zhou, S.; Tao, P.; Liu, J.
Purification and characterization of a 4-hydroxybenzoate decarboxylase from Chlamydophila pneumoniae AR39
Curr. Microbiol.
54
102-107
2007
Chlamydia pneumoniae, Chlamydia pneumoniae AR39
brenda
Lupa, B.; Lyon, D.; Shaw, L.N.; Sieprawska-Lupa, M.; Wiegel, J.
Properties of the reversible nonoxidative vanillate/4-hydroxybenzoate decarboxylase from Bacillus subtilis
Can. J. Microbiol.
54
75-81
2008
Bacillus subtilis (C0H3U9), Bacillus subtilis
brenda
Miao, L.; Li, Q.; Diao, A.; Zhang, X.; Ma, Y.
Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation
Appl. Microbiol. Biotechnol.
99
5163-5173
2015
Escherichia coli (AFH12530 and AFH12529 and AFH12528), Escherichia coli, Escherichia coli ATCC 9637 (AFH12530 and AFH12529 and AFH12528)
brenda
Sonoki, T.; Morooka, M.; Sakamoto, K.; Otsuka, Y.; Nakamura, M.; Jellison, J.; Goodell, B.
Enhancement of protocatechuate decarboxylase activity for the effective production of muconate from lignin-related aromatic compounds
J. Biotechnol.
192
71-77
2014
Klebsiella pneumoniae (X5I148), Klebsiella pneumoniae NBRC14940 (X5I148)
brenda
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