Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,4-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
2,4,6-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
2,4-Dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
3,4,5-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
NH4+ | decreases carboxylation of 3,4-dihydroxybenzoate by about 20% | Sedimentibacter hydroxybenzoicus | |
Rb+ | decreases carboxylation of 3,4-dihydroxybenzoate by about 20% | Sedimentibacter hydroxybenzoicus | |
Zn2+ | destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate | Sedimentibacter hydroxybenzoicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
4-hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
1.2 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | minor stimulation of carboxylation of 4-hydroxybenzoate | Sedimentibacter hydroxybenzoicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
6 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
350000 | - |
gradient PAGE, gel filtration | Sedimentibacter hydroxybenzoicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sedimentibacter hydroxybenzoicus | - |
Clostridium hydroxybenzoicum, strain JW/Z-1T | - |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive, 50% loss of activity when exposed to air at 5°C for 2 h | Sedimentibacter hydroxybenzoicus |
Purification (Comment) | Organism |
---|---|
- |
Sedimentibacter hydroxybenzoicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.29 | - |
- |
Sedimentibacter hydroxybenzoicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-Dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | Catechol + CO2 | - |
? | |
4-Hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | Phenol + CO2 | - |
? | |
Catechol + CO2 | - |
Sedimentibacter hydroxybenzoicus | 3,4-Dihydroxybenzoate | - |
? | |
Phenol + CO2 | - |
Sedimentibacter hydroxybenzoicus | 4-Hydroxybenzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sedimentibacter hydroxybenzoicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
11 | 40 | pH 6.0, stable for more than 24 h | Sedimentibacter hydroxybenzoicus |
50 | - |
half-life: 12 h | Sedimentibacter hydroxybenzoicus |
56 | - |
half-life: 15 min | Sedimentibacter hydroxybenzoicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.3 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
55 | - |
4-hydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6.2 | - |
Sedimentibacter hydroxybenzoicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.3 | stable for 2 h | Sedimentibacter hydroxybenzoicus |
6 | - |
11-40°C, stable for more than 24 h | Sedimentibacter hydroxybenzoicus |