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Literature summary for 3.9.1.3 extracted from
- Crystallographic characterization of a novel protein SixA which exhibits phospho-histidine phosphatase activity in the multistep His-Asp phosphorelay (1999), Acta Crystallogr. Sect. D, 55, 269-271.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, with 20 mM CaCl2, 50 mM NaCl, 0.5 mM dithiothreitol and 6% (w/v) PEG 6 K using a reservoir containing 100 mM MES-K buffer (pH 6.6), 40 mM CaCl2, 50 mM NaCl and 12% (w/v) PEG 6 K at 10°C | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P76502 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Mono-Q column chromatography, and Sephacryl S-100 gel filtration | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospho-histidine phosphatase | - |
Escherichia coli |
| SixA | - |
Escherichia coli |
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