Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Gold BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with substrates, hanging drop vapor diffusion method, using 25% (w/v) PEG3350 as precipitant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one | - |
Homo sapiens | |
2-amino-9-methyl-1,9-dihydro-6H-purin-6-one | - |
Homo sapiens | |
2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione | - |
Homo sapiens | |
3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol | - |
Homo sapiens | |
6-(methylamino)pyrimidine-2,4(1H,3H)-dione | - |
Homo sapiens | |
9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one | - |
Homo sapiens | |
N-(1-methyl-1H-benzimidazol-5-yl)acetamide | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
8-oxo-dATP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.0113 | - |
8-oxo-dGTP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.0134 | - |
2-hydroxy-rATP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.014 | - |
2-hydroxy-dATP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.082 | - |
8-oxo-rGTP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.24 | - |
8-oxo-rATP | at pH 7.4, temperature not specified in the publication | Homo sapiens | |
0.42 | - |
dGTP | at pH 7.4, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P36639 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and S75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-dATP + H2O | - |
Homo sapiens | 2-hydroxy-dAMP + diphosphate | - |
? | |
2-hydroxy-rATP + H2O | - |
Homo sapiens | 2-hydroxy-rAMP + diphosphate | - |
? | |
8-oxo-dATP + H2O | - |
Homo sapiens | 8-oxo-dAMP + diphosphate | - |
? | |
8-oxo-dGTP + H2O | - |
Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? | |
8-oxo-rATP + H2O | - |
Homo sapiens | 8-oxo-rAMP + diphosphate | - |
? | |
8-oxo-rGTP + H2O | - |
Homo sapiens | 8-oxo-rGMP + diphosphate | - |
? | |
dGTP + H2O | - |
Homo sapiens | dGMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTH1 | - |
Homo sapiens |
NUDT1 | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0015 | - |
pH and temperature not specified in the publication | Homo sapiens | 6-(methylamino)pyrimidine-2,4(1H,3H)-dione | |
0.0045 | - |
pH and temperature not specified in the publication | Homo sapiens | 3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol | |
0.059 | - |
pH and temperature not specified in the publication | Homo sapiens | 9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one | |
0.08 | - |
IC50 above 0.01 mM, pH and temperature not specified in the publication | Homo sapiens | N-(1-methyl-1H-benzimidazol-5-yl)acetamide | |
0.1 | - |
IC50 above 0.1 mM, pH and temperature not specified in the publication | Homo sapiens | 2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one | |
0.1 | - |
IC50 above 0.1 mM, pH and temperature not specified in the publication | Homo sapiens | 2-amino-9-methyl-1,9-dihydro-6H-purin-6-one | |
0.1 | - |
IC50 above 0.1 mM, pH and temperature not specified in the publication | Homo sapiens | 2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione |