Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by glucose 6-phosphate | Canis lupus familiaris | |
N-acetylglucosamine 6-phosphate | the cooperativity of the reaction is completely abolished by saturating concentrations of GlcNAcGP, this allosteric modulator activated the reaction with a typical K-effect, overview | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-amino-D-glucitol 6-phosphate | - |
Canis lupus familiaris | |
additional information | no effect by glucose 6-phosphate | Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme displays positive homotropic cooperativity towards D-glucosamine 6-phosphate | Canis lupus familiaris | |
0.25 | - |
D-glucosamine 6-phosphate | hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30400 | - |
6 * 30400, SDS-PAGE | Canis lupus familiaris |
180000 | - |
about, gel filtration | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | Canis lupus familiaris | - |
D-fructose 6-phosphate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 1390fold from kidney cortex by affinity chromatography on N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose and on phosphocellulose, the latter behaving as an active site affinity resin | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | cortex | Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
purified enzyme | Canis lupus familiaris |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, pH 5.0-9.0, overnight, in presence of different salts, stable | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | - |
Canis lupus familiaris | D-fructose 6-phosphate + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 30400, SDS-PAGE | Canis lupus familiaris |
Synonyms | Comment | Organism |
---|---|---|
More | formerly EC 5.3.1.10 | Canis lupus familiaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
D-glucosamine 6-phosphate | per subunit, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris | |
255 | - |
D-glucosamine 6-phosphate | hexameric enzyme, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Canis lupus familiaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH-profile, overview | Canis lupus familiaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 4°C, purified enzyme, overnight, in presence of different salts, stable | Canis lupus familiaris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Canis lupus familiaris | isoelectric focusing and chromatofocusing | - |
5.7 |