Cloned (Comment) | Organism |
---|---|
overexpressed as an N-terminally His6 tagged protein in Bl21(DE3) cells | Neisseria gonorrhoeae |
Crystallization (Comment) | Organism |
---|---|
crystals are grown at 20°C by vapor diffusion in sitting drops. High-resolution crystal structures of the enzyme: one in complex with the reaction intermediate analog and competitive inhibitor 8-oxo-GTP, and one with a TRIS molecule bound in the active site and mimicking another reaction intermediate | Neisseria gonorrhoeae |
Protein Variants | Comment | Organism |
---|---|---|
C149A | mutant enzyme shows no activity | Neisseria gonorrhoeae |
C149A | mutant protein purified under the same conditions as wild-type enzyme lacks any S-nitrosylated thiols. S-nitrosylation of the wild-type enzyme occurs posttranslationally during heterologous expression in Escherichia coli and that it is specific to Cys149 | Neisseria gonorrhoeae |
C149S | mutant enzyme exhibits 2.7% of the wild-type activity | Neisseria gonorrhoeae |
E152A | mutant enzyme exhibits about 20% of the wild-type activity | Neisseria gonorrhoeae |
E243A | mutant enzyme exhibits 0.37% of the wild-type activity | Neisseria gonorrhoeae |
H246A | mutant enzyme exhibits 0.74% of the wild-type activity | Neisseria gonorrhoeae |
H246D | mutant enzyme exhibits 3.0% of the wild-type activity | Neisseria gonorrhoeae |
H246K | mutant enzyme shows no activity | Neisseria gonorrhoeae |
H246N | mutant enzyme shows no activity | Neisseria gonorrhoeae |
H246Q | mutant enzyme exhibits 2.4% of the wild-type activity | Neisseria gonorrhoeae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-GTP | - |
Neisseria gonorrhoeae | |
7-deaza-GTP | - |
Neisseria gonorrhoeae | |
8-oxo-GTP | competitive inhibitor. The inhibitor interacts with a conserved active site Cys149, and this residue is S-nitrosylated. Ki/Km: 0.022 | Neisseria gonorrhoeae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00915 | - |
GTP | pH and temperature not specified in the publication | Neisseria gonorrhoeae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+-dependent enzyme | Neisseria gonorrhoeae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
gel filtration | Neisseria gonorrhoeae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Neisseria gonorrhoeae | - |
formate + 7,8-dihydroneopterin 3'-triphosphate | - |
? | |
GTP + H2O | Neisseria gonorrhoeae ATCC 700825 | - |
formate + 7,8-dihydroneopterin 3'-triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | Q5F9K6 | - |
- |
Neisseria gonorrhoeae ATCC 700825 | Q5F9K6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Neisseria gonorrhoeae | formate + 7,8-dihydroneopterin 3'-triphosphate | - |
? | |
GTP + H2O | - |
Neisseria gonorrhoeae ATCC 700825 | formate + 7,8-dihydroneopterin 3'-triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Neisseria gonorrhoeae |
Synonyms | Comment | Organism |
---|---|---|
FelE | - |
Neisseria gonorrhoeae |
GCYH-IB | - |
Neisseria gonorrhoeae |
GTP cyclohydrolase IB | - |
Neisseria gonorrhoeae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000265 | - |
GTP | pH and temperature not specified in the publication | Neisseria gonorrhoeae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000149 | - |
8-oxo-GTP | pH and temperature not specified in the publication | Neisseria gonorrhoeae | |
0.0457 | - |
7-deaza-GTP | pH and temperature not specified in the publication | Neisseria gonorrhoeae | |
9.164 | - |
2'-deoxy-GTP | pH and temperature not specified in the publication | Neisseria gonorrhoeae |