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Literature summary for 3.4.22.69 extracted from

  • Kuo, C.J.; Liang, P.H.
    SARS-CoV-2 3CLpro displays faster self-maturation in vitro than SARS-CoV 3CLpro due to faster C-terminal cleavage (2022), FEBS Lett., 596, 1214-1224 .
    View publication on PubMed
Search for more literature for 3.4.22.69

Protein Variants

Protein Variants Comment Organism
C145A catalytically inactive Severe acute respiratory syndrome coronavirus 2
C145A catalytically inactive severe acute respiratory syndrome coronavirus

Organism

Organism UniProt Comment Textmining
severe acute respiratory syndrome coronavirus
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-
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Severe acute respiratory syndrome coronavirus 2 P0DTD1 replicase polyprotein 1ab
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SARSCoV 3CLpro favors Ala and Ser, followed by Gly at the P1' position severe acute respiratory syndrome coronavirus ?
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additional information the N-terminal cleavage site G-S of substrate is preferred over the C-terminal cleavage site Q-G Severe acute respiratory syndrome coronavirus 2 ?
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-
SARS-CoV Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive Severe acute respiratory syndrome coronavirus 2 Trx-10aa + C145A 3CLpro mutant + 10aa-GST
-
 ?
SARS-CoV Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive severe acute respiratory syndrome coronavirus Trx-10aa + C145A 3CLpro mutant + 10aa-GST
-
 ?
SARS-CoV-2 Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive Severe acute respiratory syndrome coronavirus 2 Trx-10aa + C145A 3CLpro mutant + 10aa-GST
-
 ?
SARS-CoV-2 Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive severe acute respiratory syndrome coronavirus Trx-10aa + C145A 3CLpro mutant + 10aa-GST
-
 ?

General Information

General Information Comment Organism
metabolism in full-length inactive 3CLpro with N- and C-terminal extensions as substrates for monitoring self-cleavage, the rate-limiting C-terminal self-cleavage rate of SARS-CoV-2 3CLpro is 35fold faster than that of SARS-CoV 3CLpro using Trx/GST-tagged C145A 3CLpro substrates Severe acute respiratory syndrome coronavirus 2
metabolism in full-length inactive 3CLpro with N- and C-terminal extensions as substrates for monitoring self-cleavage, the rate-limiting C-terminal self-cleavage rate of SARS-CoV-2 3CLpro is 35fold faster than that of SARS-CoV 3CLpro using Trx/GST-tagged C145A 3CLpro substrates severe acute respiratory syndrome coronavirus