Crystallization (Comment) | Organism |
---|---|
crystallization of free enzyme and the enzyme/peptide aldehyde inhibitor(benzoyl-YYNQ-H) complex by hanging-drop method. The 3CLpro structure (refined to 1.94 A) shows that the protein forms dimers. The monomers are comprised of N-terminal domains I and II, which adopt a chymotrypsin-like fold, and a C-terminal alpha-helical domain III. The catalytic Cys-His dyad is assisted by a complex network of interactions involving a water molecule that mediates polar contacts between the catalytic His and a conserved Asp located in the domain II-III junction and is suitably positioned to stabilize the developing positive charge of the catalytic His in the transition state during catalysis | Alphamesonivirus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoyl-YYNQ-H | - |
Alphamesonivirus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alphamesonivirus 1 | F8RL29 | isolate Aedes harrisoni | - |
Purification (Comment) | Organism |
---|---|
- |
Alphamesonivirus 1 |
Synonyms | Comment | Organism |
---|---|---|
3C-like cysteine protease | - |
Alphamesonivirus 1 |