Cloned (Comment) | Organism |
---|---|
gene Lyccasp9, DNA and maino acid sequence determination and analysis, full-length cDNA of Lyccasp9 is 2595 bp with an open reading frame of 1314 bp encoding a polypeptide of 437 amino acids, which includes a 90-amino acids caspase recruitment domain (CARD, residues 1-90), a 133-amino acids p20 domain (residues 171-303) with two putative caspase family histidine and cysteine active sites, as well as an 87-amino acids p10 domain (residues 348-435) | Larimichthys crocea |
Protein Variants | Comment | Organism |
---|---|---|
C299G | site-directed mutagenesis, the mutant displays significantly decreased proteolytic activity compared to the wild-type enzyme | Larimichthys crocea |
H249D | site-directed mutagenesis, the mutant displays significantly decreased proteolytic activity compared to the wild-type enzyme | Larimichthys crocea |
L228V | site-directed mutagenesis | Larimichthys crocea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Larimichthys crocea | - |
gene Lyccasp9 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | high expression level | Larimichthys crocea | - |
gill | - |
Larimichthys crocea | - |
heart | high expression level | Larimichthys crocea | - |
intestine | - |
Larimichthys crocea | - |
kidney | - |
Larimichthys crocea | - |
liver | - |
Larimichthys crocea | - |
additional information | Lyccasp9 is constitutively ubiquitously expressed, although expression levels vary from tissue to tissue, real-time PCR expression analysis | Larimichthys crocea | - |
muscle | - |
Larimichthys crocea | - |
spleen | - |
Larimichthys crocea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-LEDH-4-nitroanilide + H2O | - |
Larimichthys crocea | Ac-LEDH + 4-nitroaniline | - |
? | |
additional information | catalytic Cys299 residue with a His249 involved in catalysis | Larimichthys crocea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the caspase family signature histidine active-site H236SQYDCCVVIMLSHG250, the cysteine active-site K290PKLFFIQACGG301 and the caspase 9 characteristic pentapeptide active-site QACGG are located in the p20 domain | Larimichthys crocea |
Synonyms | Comment | Organism |
---|---|---|
caspase 9 | - |
Larimichthys crocea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Larimichthys crocea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Larimichthys crocea |
General Information | Comment | Organism |
---|---|---|
evolution | multiple sequence alignment and phylogenetic tree show that fish and human caspase 9 are highly evolutionarily conserved | Larimichthys crocea |
additional information | the caspase family signature histidine active-site H236SQYDCCVVIMLSHG250, the cysteine active-site K290PKLFFIQACGG301 and the caspase 9 characteristic pentapeptide active-site QACGG are located in the p20 domain | Larimichthys crocea |