Activating Compound | Comment | Organism | Structure |
---|---|---|---|
harmol | i.e. 1-methyl-9H-beta-carbolin-7-ol, a natural beta-carboline plant alkaloid, induces caspase-8 activation | Homo sapiens | |
additional information | no enzyme activation by natural beta-carboline alkaloids harmalol, harmine, and harmaline, i.e. 1-methyl-4,9-dihydro-3H-beta-carbolin-7-ol, 7-methoxy-1-methyl-9H-beta-carboline, and 7-methoxy-1-methyl-4,9-dihydro-3H-beta-carboline, respectively | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-LEHD-fluoromethylketone | a caspase-9 inhibitor, partial inhibition of caspase-8 activation in lung carcinoma cells | Homo sapiens | |
N-carbobenzyloxy-IETD-fluoromethyl ketone | a caspase-8 inhibitor, inhibition of caspase-8 activation in lung carcinoma cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | harmol activates caspase-8 to 334% and induces apoptosis by caspase-8 activation independently on Fas/Fas ligand interaction in human lung carcinoma H-596 cells, but not in H-596, H-226, and A-549 cells | ? | - |
? | |
procaspase-3 + H2O | Homo sapiens | activation of effector caspase-3 by the initiator caspase-8 | caspase-3 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung carcinoma cell | - |
Homo sapiens | - |
NCI-H596 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-IETD-7-amido-4-fluoromethylcoumarin + H2O | - |
Homo sapiens | acetyl-IETD + 7-amino-4-fluoromethylcoumarin | - |
? | |
additional information | harmol activates caspase-8 to 334% and induces apoptosis by caspase-8 activation independently on Fas/Fas ligand interaction in human lung carcinoma H-596 cells, but not in H-596, H-226, and A-549 cells | Homo sapiens | ? | - |
? | |
procaspase-3 + H2O | activation | Homo sapiens | caspase-3 + ? | - |
? | |
procaspase-3 + H2O | activation of effector caspase-3 by the initiator caspase-8 | Homo sapiens | caspase-3 + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |