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Literature summary for 3.4.22.43 extracted from

  • Yasuda, Y.; Li, Z.; Greenbaum, D.; Bogyo, M.; Weber, E.; Broemme, D.
    Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages (2004), J. Biol. Chem., 279, 36761-36770.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
chondroitin 4-sulfate specific inhibition of elastolytic activity, formation of specific complexes with the enzyme, no inhibition of the activity with Z-Phe-Arg-4-methyl-7-coumaryl amide Homo sapiens
E-64
-
Homo sapiens
morpholineurea-leucyl-homophenylalanine-vinylsulfone phenyl
-
Homo sapiens
morpholineurea-naphthyl-homophenylalanine-vinylsulfone naphthyl
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
Z-Phe-Arg-4-methyl-7-coumaryl amide recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl Homo sapiens
0.0048
-
Z-Phe-Arg-4-methyl-7-coumaryl amide recombinant enzyme, pH 5.5, 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Homo sapiens 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl stimulates at 0.3 M Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Elastin + H2O Homo sapiens elastin degradation activity leading to loss of elasticity in atherosclerosis ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the recombinant enzyme is activated by pepsin treatment and further purified by gel filtration Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris, further purified by gel filtration after activation by pepsin treatment Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell
-
Homo sapiens
-
cornea
-
Homo sapiens
-
macrophage in plaque areas of diseased blood vessels, cathepsin expression levels during differentiation, overview Homo sapiens
-
testis
-
Homo sapiens
-
thymus
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Elastin + H2O elastin degradation activity leading to loss of elasticity in atherosclerosis Homo sapiens ?
-
?
Elastin + H2O the enzyme exhibits high elastolytic cysteine protease activity Homo sapiens ?
-
?
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O
-
Homo sapiens Z-Arg-Arg + 4-methoxy-2-nitroaniline
-
?
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
-
Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 5.5, loss of 60% activity within 20 min Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
Z-Phe-Arg-4-methyl-7-coumaryl amide recombinant enzyme, pH 5.5, 25°C Homo sapiens
3.7
-
Z-Phe-Arg-4-methyl-7-coumaryl amide recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
37°C, loss of 60% activity within 20 min Homo sapiens