Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chondroitin 4-sulfate | specific inhibition of elastolytic activity, formation of specific complexes with the enzyme, no inhibition of the activity with Z-Phe-Arg-4-methyl-7-coumaryl amide | Homo sapiens | |
E-64 | - |
Homo sapiens | |
morpholineurea-leucyl-homophenylalanine-vinylsulfone phenyl | - |
Homo sapiens | |
morpholineurea-naphthyl-homophenylalanine-vinylsulfone naphthyl | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
Z-Phe-Arg-4-methyl-7-coumaryl amide | recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl | Homo sapiens | |
0.0048 | - |
Z-Phe-Arg-4-methyl-7-coumaryl amide | recombinant enzyme, pH 5.5, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Homo sapiens | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | stimulates at 0.3 M | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Elastin + H2O | Homo sapiens | elastin degradation activity leading to loss of elasticity in atherosclerosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant enzyme is activated by pepsin treatment and further purified by gel filtration | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris, further purified by gel filtration after activation by pepsin treatment | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
cornea | - |
Homo sapiens | - |
macrophage | in plaque areas of diseased blood vessels, cathepsin expression levels during differentiation, overview | Homo sapiens | - |
testis | - |
Homo sapiens | - |
thymus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Elastin + H2O | elastin degradation activity leading to loss of elasticity in atherosclerosis | Homo sapiens | ? | - |
? | |
Elastin + H2O | the enzyme exhibits high elastolytic cysteine protease activity | Homo sapiens | ? | - |
? | |
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O | - |
Homo sapiens | Z-Arg-Arg + 4-methoxy-2-nitroaniline | - |
? | |
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O | - |
Homo sapiens | Z-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 5.5, loss of 60% activity within 20 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
Z-Phe-Arg-4-methyl-7-coumaryl amide | recombinant enzyme, pH 5.5, 25°C | Homo sapiens | |
3.7 | - |
Z-Phe-Arg-4-methyl-7-coumaryl amide | recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
37°C, loss of 60% activity within 20 min | Homo sapiens |