Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.43 extracted from

  • Broemme, D.
    Cathepsin V (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd Ed., 1107-1109.
No PubMed abstract available

Application

Application Comment Organism
diagnostics the enzyme is a potential marker for certain colon tumors and breast cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
from cDNA libraries, the gene maps to chromosome 9q21, DNA and amino acid sequence determination and analysis, genomic organization, expression in insect cells using the baculovirus system, in Pichia pastoris, and as GST-tagged enzyme with low activity in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
calpeptin
-
Homo sapiens
cystatins
-
Homo sapiens
E-64
-
Homo sapiens
leupeptin
-
Homo sapiens
peptidl vinyl sulfones
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23999
-
2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris Homo sapiens
31000
-
2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris Homo sapiens
52000
-
recombinant GST-tagged enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation of extracellular proenzyme and mature enzyme Homo sapiens
proteolytic modification autoactivation of the inactive extracellular zymogen at pH 4.0 and 37°C to the active mature protein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris to homogeneity, activated mature native enzyme by hyrophobic interaction chromatography, recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cornea epithelium Homo sapiens
-
epithelium thymic and corneal Homo sapiens
-
additional information selective, tissue-specific expression Homo sapiens
-
testis
-
Homo sapiens
-
thymus epithelium Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O
-
Homo sapiens ?
-
?
MHC class II-associated invariant chain + H2O
-
Homo sapiens ?
-
?
additional information the enzyme prefers hydrophobic residues in its S2-binding pocket, the substrate specificity is similar to cathepsin L, no cleavage of the triple-helical region of native collagen Homo sapiens ?
-
?
Type I collagen + H2O cleavage of telopeptide region Homo sapiens ?
-
?
Z-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Z-Leu-Arg + 7-amino-4-methylcoumarin
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris Homo sapiens
More three-dimensional two-domain structure analysis at 1.6 A resolution, overview Homo sapiens

Synonyms

Synonyms Comment Organism
cathepsin L2
-
Homo sapiens
cathepsin U
-
Homo sapiens
More the enzyme belongs to the C1A peptidase family Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
bell-shaped pH profile Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
rapid loss of activity at neutral pH Homo sapiens
4.5 5.5 very stable Homo sapiens