Inhibitors | Comment | Organism | Structure |
---|---|---|---|
o-aminobenzoyl-F-4-(aminomethyl)cyclohexylalanine-SRQ-N-(2,4-dinitrophenyl)ethylene diamine | K1, highly specific | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.00064 | - |
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.0007 | - |
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.0007 | - |
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.001 | - |
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.0036 | - |
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.0038 | - |
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Preferential cleavage of Arg-/- bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-/- or Leu-/-. The rat enzyme is unusual in liberating bradykinin directly from autologous kininogens by cleavage at two Arg-/- bonds | S1 subsite accepts both Arg and Phe and has a preference for basic residues | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
urine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
low-molecular-weight kininogen + H2O | - |
Homo sapiens | kallidin + kinin | kallidin is Lys-bradykinin | ? | |
additional information | no activity with substrate o-aminobenzoyl-FXSRQ-N-(2,4-dinitrophenyl)ethylene diamine harboring at position X a non-natural amino acid like 4-guanidine phenylalanine, 3-pyridylalanine, 4-(aminomethyl)cyclohexylalanine, 4-(aminomethyl)-N-isopropylphenylalanine, 4-piperidinylalanine, and 4-(aminocyclohexyl)alanine | Homo sapiens | ? | - |
? | |
additional information | K1, sustrate specificty of S1 subsite, accepts both Arg and Phe | Homo sapiens | ? | - |
? | |
additional information | no activity with substrate o-aminobenzoyl-GFSPFXSSRPQ-N-(2,4-dinitrophenyl)ethylene diamine harboring at position X a non-natural amino acid like 4-guanidine phenylalanine, 3-pyridylalanine, and 4-(aminomethyl)cyclohexylalanine | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O | synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin | Homo sapiens | o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
polypeptide + H2O | - |
Homo sapiens | peptides | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.61 | - |
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
0.81 | - |
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
1 | - |
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
1.2 | - |
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
1.8 | - |
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens | |
2.1 | - |
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
0.00005 | - |
o-aminobenzoyl-F-4-(aminomethyl)cyclohexylalanine-SRQ-N-(2,4-dinitrophenyl)ethylene diamine | pH 9.0, 37°C | Homo sapiens |