Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | stimulation | Vibrio sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module | Vibrio sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module. The catalytic module alone exhibits 50fold higher acivity than the full-length enzyme | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1mM, complete inhibition | Vibrio sp. | |
CuCl2 | 1mM, complete inhibition | Vibrio sp. | |
CuSO4 | 1mM, complete inhibition | Vibrio sp. | |
HgCl2 | 1mM, complete inhibition | Vibrio sp. | |
Pb(CH3COO)2 | 1mM, complete inhibition | Vibrio sp. | |
sodium dodecylsulfate | 0.1%, complete inhibition | Vibrio sp. | |
ZnCl2 | 1mM, complete inhibition | Vibrio sp. | |
ZnSO4 | 1mM, complete inhibition | Vibrio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value of the catalytic module for agarose is 2.33 mg/ml | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50824 | - |
x * 50824, calculated | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
wild-type | Vibrio sp. |
63.6 | - |
catalytic module | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Vibrio sp. | neoagarotetraose | plus small amounts of neoagarohexaose and neoagarobiose | ? | |
additional information | no substrate: neoagarotetraose, kappa-carrageenan | Vibrio sp. | ? | - |
? | |
neoagarohexaose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarobiose | - |
? | |
porphyran + H2O | - |
Vibrio sp. | neoagaro-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50824, calculated | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
AgaD | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, stable | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 12 h, 25°C, retains more than 80% of activity | Vibrio sp. |