Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme in Saccharomyces cerevisiae strain C468, cloning and expression of mutant enzymes in Escherichia coli strain DH5alpha | Aspergillus awamori |
Protein Variants | Comment | Organism |
---|---|---|
A246C | site-directed mutagenesis, the mutant has a strong additive thermostabilizing effect | Aspergillus awamori |
G127A/P128A | site-directed mutagenesis, the mutation decreases the enzyme thermostability compared to the wild-type protein | Aspergillus awamori |
G137A | site-directed mutagenesis, the mutant has a strong additive thermostabilizing effect | Aspergillus awamori |
G139A | site-directed mutagenesis, the mutant has a strong additive thermostabilizing effect | Aspergillus awamori |
I136L | site-directed mutagenesis, the mutant has a strong additive thermostabilizing effect | Aspergillus awamori |
additional information | molecular construction, molecular modeling and molecular dynamics of engineered enzyme with higher thermostability through optimized intrinsic interactions within alpha-helix D, overview | Aspergillus awamori |
P128A | site-directed mutagenesis, the mutant destabilizes the enzyme | Aspergillus awamori |
P128A/G139A/I136L | site-directed mutagenesis, mutations G139A and I136L, located in the center of alpha-helix, completely compensate for the destabilization caused by substitution P128A | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus awamori X100 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the hydrolysis reaction successively cleaves glucose residues from the nonreducing ends of starch, glycogen, and maltooligosaccharides | Aspergillus awamori | ? | - |
? | |
additional information | the hydrolysis reaction successively cleaves glucose residues from the nonreducing ends of starch, glycogen, and maltooligosaccharides | Aspergillus awamori X100 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Aspergillus awamori |