Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, constitutive overexpression of the His-tagged enzyme in Escherichia coli | Clostridium thermoamylolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
maltose | pH 4.5, 65°C, recombinant enzyme | Clostridium thermoamylolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation | Clostridium thermoamylolyticum |
79920 | - |
x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation | Clostridium thermoamylolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium thermoamylolyticum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 10fold from Escherichia coli by nickel affinity chromatography | Clostridium thermoamylolyticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
purified recombinant enzyme | Clostridium thermoamylolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Clostridium thermoamylolyticum | beta-D-glucose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation | Clostridium thermoamylolyticum |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Clostridium thermoamylolyticum |
More | the enzyme belongs to the glycoside hydrolase family 15 | Clostridium thermoamylolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Clostridium thermoamylolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
80% remaining activity | Clostridium thermoamylolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
maltose | pH 4.5, recombinant enzyme | Clostridium thermoamylolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant enzyme | Clostridium thermoamylolyticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.9 | 9 | over 80% at pH 3.9 and pH 9.0 | Clostridium thermoamylolyticum |