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Literature summary for 3.2.1.209 extracted from

  • Mora-Montes, H.M.; Bader, O.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Hube, B.; Gow, N.A.; Flores-Carreon, A.
    Kex2 protease converts the endoplasmic reticulum alpha1,2-mannosidase of Candida albicans into a soluble cytosolic form (2008), Microbiology, 154, 3782-3794.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.209

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Candida albicans
swainsonine
-
 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol E-I Candida albicans 5829
-
cytosol isoform E-I Candida albicans 5829
-
endoplasmic reticulum 65000 Da membrane-bound isoform Candida albicans 5783
-
endoplasmic reticulum membrane
-
 Candida albicans 5789
-
Golgi vesicle
-
 Candida albicans
-
-
Golgi vesicle presence of a 65000 Da membrane-bound isoform that is cleaved by protease Kex2 to soluble 52000 Da isoform E-I Candida albicans
-
-
membrane
-
 Candida albicans 16020
-
soluble
-
 Candida albicans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 E-I, cytosoloc fraction, Golgi-derived vesicles, SDS-PAGE Candida albicans
52000
-
 gel fitlration, isoform E-I Candida albicans
65000
-
 membrane bound, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Candida albicans cytosolic isoform E-I Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Candida albicans ATCC 26555 cytosolic isoform E-I Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans Q8J0Q0
-
-
Candida albicans Q8J0Q0 strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
-
Candida albicans ATCC 26555 Q8J0Q0 strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification converting via Kex2-protease Candida albicans

Purification (Commentary)

Purification (Comment) Organism
-
 Candida albicans

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0908
-
 Candida albicans kex-2-delta-mutant Candida albicans
0.0946
-
 Candida albicans strain CAI4 Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + alpha-D-mannose
-
 ?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans ATCC 26555 4-methylumbelliferone + alpha-D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O cytosolic isoform E-I Candida albicans Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O cytosolic isoform E-I Candida albicans ATCC 26555 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
additional information cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B Candida albicans ?
-
 ?
additional information cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B Candida albicans ATCC 26555 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE, isoform E-I Candida albicans

Synonyms

Synonyms Comment Organism
alpha1,2-mannosidases
-
 Candida albicans
endoplasmic reticulum alpha1,2-mannosidase
-
 Candida albicans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.22
-
-
 Candida albicans 1-deoxymannojirimycin
0.54
-
-
 Candida albicans swainsonine