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Literature summary for 3.2.1.207 extracted from

  • Watanabe, S.; Kakudo, A.; Ohta, M.; Mita, K.; Fujiyama, K.; Inumaru, S.
    Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda (2013), Insect Biochem. Mol. Biol., 43, 319-327 .
    View publication on PubMed
Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment) Organism
-
 Spodoptera frugiperda
-
 Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.37
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.5, 37°C Spodoptera frugiperda
2.82
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.5, 37°C Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori L8B8U1 and L8B6C5 L8B8U1 i.e. alpha subunit, L8B6C5 i.e. beta subunit
-
Spodoptera frugiperda L8B8V1 alpha subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.53
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 6.5, 37°C Bombyx mori
4.65
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 6.5, 37°C Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Bombyx mori 4-nitrophenol + D-glucose
-
 ?
GlcMan9GlcNAc2-pyridylamine + H2O
-
 Spodoptera frugiperda Man9GlcNAc2-pyridylamine + D-glucose
-
 ?
GlcMan9GlcNAc2-pyridylamine + H2O
-
 Bombyx mori Man9GlcNAc2-pyridylamine + D-glucose
-
 ?
N-glycan + H2O
-
 Spodoptera frugiperda ? + D-glucose
-
 ?
N-glycan + H2O
-
 Bombyx mori ? + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 104633, calculated for alpha subunit, plus x* 59514, calculated for beta subunit Bombyx mori
? x * 105206, calculated for alpha subunit Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Spodoptera frugiperda
6.5
-
-
 Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
 Spodoptera frugiperda
4 11
-
 Bombyx mori