Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.207 extracted from

  • Olson, L.J.; Orsi, R.; Alculumbre, S.G.; Peterson, F.C.; Stigliano, I.D.; Parodi, A.J.; DAlessio, C.; Dahms, N.M.
    Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum (2013), J. Biol. Chem., 288, 16460-16475.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment) Organism
beta-subunit, DNA and amino acid sequence determination and analysis, recombinant expression as His-tagged protein in Escherichia coli strain BL21(pREP4) Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
W409A site-directed mutagenesis of the beta subunit MRH domain, the mutant shows altered ligand binding activity compared to the wild-type beta-subunit Schizosaccharomyces pombe
W409F site-directed mutagenesis of the beta subunit MRH domain, the mutant shows altered ligand binding activity compared to the wild-type beta-subunit Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Schizosaccharomyces pombe 5783
-
additional information the enzyme contains a signal peptide (first 23 amino acids) sequence Schizosaccharomyces pombe
-
-

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged beta-subunit by immobilized metal affinity chromatography from Escherichia coli strain BL21(pREP4) Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information interaction of the beta-subunit MRH domain with mannosyl-alpha-(1,2)-mannose, mannose 6-phosphate, mannosyl-N-acetylglucsamine, or glucose 6-phosphate, ligand binding structures, overview Schizosaccharomyces pombe ?
-
 ?

Synonyms

Synonyms Comment Organism
glucosidase II
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
additional information the inability of beta-subunit MRH domain to efficiently recognize glucose eliminates the potential for the beta-subunit to compete with the catalytic alpha-subunit of GII for glucose present in arm A of the N-glycan, ligand binding structures, overview. Possible models for the influence of Trp409 in enzyme activity Schizosaccharomyces pombe
physiological function the enzyme is involved in quality control of glycoprotein folding in the endoplasmic reticulum, overview Schizosaccharomyces pombe